Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-12-5
pubmed:abstractText
Twelve monoclonal antibodies have been raised against proteins in preparations of Z-disks isolated from Drosophila melanogaster flight muscle. The monoclonal antibodies that recognized Z-band components were identified by immunofluorescence microscopy of flight muscle myofibrils. These antibodies have identified three Z-disk antigens on immunoblots of myofibrillar proteins. Monoclonal antibodies alpha:1-4 recognize a 90-100-kD protein which we identify as alpha-actinin on the basis of cross-reactivity with antibodies raised against honeybee and vertebrate alpha-actinins. Monoclonal antibodies P:1-4 bind to the high molecular mass protein, projectin, a component of connecting filaments that link the ends of thick filaments to the Z-band in insect asynchronous flight muscles. The anti-projectin antibodies also stain synchronous muscle, but, surprisingly, the epitopes here are within the A-bands, not between the A- and Z-bands, as in flight muscle. Monoclonal antibodies Z(210):1-4 recognize a 210-kD protein that has not been previously shown to be a Z-band structural component. A fourth antigen, resolved as a doublet (approximately 400/600 kD) on immunoblots of Drosophila fibrillar proteins, is detected by a cross reacting antibody, Z(400):2, raised against a protein in isolated honeybee Z-disks. On Lowicryl sections of asynchronous flight muscle, indirect immunogold staining has localized alpha-actinin and the 210-kD protein throughout the matrix of the Z-band, projectin between the Z- and A-bands, and the 400/600-kD components at the I-band/Z-band junction. Drosophila alpha-actinin, projectin, and the 400/600-kD components share some antigenic determinants with corresponding honeybee proteins, but no honeybee protein interacts with any of the Z(210) antibodies.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-3086876, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-3126498, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-3301868, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-378987, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-3805258, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-3902856, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-3995053, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-4046026, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-4214935, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-4326772, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-4568399, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-4870966, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-5127429, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-6219389, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-6754746, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-6783638, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-6802813, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-6802982, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-6811687, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-6896900, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-7072935, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-7130272, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-7236199, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-7287670, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-743226, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-849268, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-926177, http://linkedlifedata.com/resource/pubmed/commentcorrection/2509482-97355
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2157-67
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Characterization of components of Z-bands in the fibrillar flight muscle of Drosophila melanogaster.
pubmed:affiliation
Department of Physiology, Boston University School of Medicine, Massachusetts 02118.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't