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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1989-10-12
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pubmed:abstractText |
Small, round-structured virus (SRSV) was detected in a stool specimen of a patient during an acute gastroenteritis outbreak in Tokyo and was tentatively named SRSV-9. SRSV-9 was purified by sucrose velocity gradient centrifugation after CsCl density gradient centrifugation. The buoyant density of SRSV-9 appeared to be 1.36 g/ml in CsCl. A Western blot (immunoblot) assay using the biotin-avidin system revealed that SRSV-9 was antigenically related to the Hawaii agent but distinct from the Norwalk agent and contained a single major structural protein with a molecular size of 63.0 +/- 0.6 kilodaltons. The prevalence of SRSV-9 infection in Tokyo was surveyed by the Western blot antibody assay by using a crude virus preparation as the antigen. Seroconversion was observed in 56.5% of the patients involved in the outbreaks from which SRSV was detected by electron microscopy.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-2786130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-2821127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-2993353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-2997382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-3000350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-3005857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-3009701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-3009862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-3020085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-3036438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-401851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-405590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-4117963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-514174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-6286787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-6286860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-6288563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-6330511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-6651531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-6785451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-6799614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-94599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504773-97365
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1728-33
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2504773-Acute Disease,
pubmed-meshheading:2504773-Antibodies, Viral,
pubmed-meshheading:2504773-Blotting, Western,
pubmed-meshheading:2504773-Centrifugation, Density Gradient,
pubmed-meshheading:2504773-Disease Outbreaks,
pubmed-meshheading:2504773-Feces,
pubmed-meshheading:2504773-Food Microbiology,
pubmed-meshheading:2504773-Gastroenteritis,
pubmed-meshheading:2504773-Humans,
pubmed-meshheading:2504773-Microscopy, Electron,
pubmed-meshheading:2504773-Tokyo,
pubmed-meshheading:2504773-Virion,
pubmed-meshheading:2504773-Virus Diseases,
pubmed-meshheading:2504773-Viruses, Unclassified
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pubmed:year |
1989
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pubmed:articleTitle |
Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo.
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pubmed:affiliation |
Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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