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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-9-25
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pubmed:abstractText |
Exposure of cultured human peripheral blood monocytes (PBMO) to interferon-gamma (IFN-gamma) enhances surface expression of the p55 subunit of the interleukin-2 receptor (IL-2R). Addition of IL-2 to IFN-gamma treated monocytes will stimulate subsequent release of interleukin-1 beta (IL-1 beta) and tumour necrosis factor-alpha (TNF-alpha) due to enhancement of the transcriptional rate and stability of mRNA accumulation of both genes. The present study was aimed to investigate whether IFN-gamma and IL-2 will favour the release of IL-1 beta and TNF-alpha by different subsets of human PBMO. To this end PBMO were separated into four fractions with densities ranging from 1.058 to 1.075 kg/l by means of discontinuous bovine serum albumin (BSA) gradient centrigugation. Following incubation of monocyte subsets in the presence or absence of IFN-gamma and IL-2 for a culture period of 24-48 h, cell-free culture supernatants were collected and assayed for IL-1 beta and TNF-alpha activity by ELISA. Surface p55 IL-2R expression was detected by CD25 monoclonal antibody (MoAb) anti-IL-2R1 using immunofluorescence analysis. Although IL-2R1 expressing PBMO were equally distributed among the four fractions, IL-1 beta secretion was found to reside mainly in the most dense fraction. The major TNF-alpha activity was detected, however, in supernatants obtained from cultures of PBMO with densities of 1.065-1.075 kg/l. These data demonstrate functional heterogeneity of subsets of human PBMO with respect to their capacity to produce distinct cytokines.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-2452833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-2467700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-2474236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-2920210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-3010124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-3102073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-3102253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-3138311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-3262679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-3499192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-6603982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-6606726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2504521-6806370
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
97-100
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2504521-Cells, Cultured,
pubmed-meshheading:2504521-Humans,
pubmed-meshheading:2504521-Interferon-gamma,
pubmed-meshheading:2504521-Interleukin-1,
pubmed-meshheading:2504521-Interleukin-2,
pubmed-meshheading:2504521-Monocytes,
pubmed-meshheading:2504521-Receptors, Interleukin-2,
pubmed-meshheading:2504521-Recombinant Proteins,
pubmed-meshheading:2504521-Tumor Necrosis Factor-alpha
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pubmed:year |
1989
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pubmed:articleTitle |
Interleukin-2 and interferon-gamma recruit different subsets of human peripheral blood monocytes to secrete interleukin-1 beta and tumour necrosis factor-alpha.
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pubmed:affiliation |
Department of Haematology, Johannes Gutenberg-University of Mainz, FRG.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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