Linked Life Data

2493743

Source:http://linkedlifedata.com/resource/pubmed/id/2493743

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 1
pubmed:dateCreated
1989-4-14
pubmed:abstractText
We investigated the pH recovery mechanisms in rabbit parietal, chief, and surface cells during pH shifts induced by introduction or removal of exogenous CO2-HCO3-. Intracellular pH (pHi) was measured using the fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescin (BCECF). Gastric cells were highly purified by density gradient centrifugation and elutriation. When cells suspended in N-2-hydroxyethylpiperazene-N'-2-ethanesulfonic acid (HEPES)-100% O2, extracellular pH (pHo) 7.4, were exposed to 24 mM HCO3- -5% CO2, pHo 7.4, all cells quickly acidified by 0.3-0.4 pH units. Almost complete pH-recovery occurred within 15 min. In parietal cells, 70% of this recovery was dependent on the presence of extracellular Na+ (Nao+) and was blocked by 1 mM amiloride. The Na+-independent recovery was blocked by intracellular Cl- depletion or by 0.4 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). In chief cells and surface cells no recovery occurred in the absence of NaO+, and 1 mM amiloride blocked pH recovery in Na+-containing buffer. On removal of HCO3- -CO2, the cells alkalinized, and subsequent pH recovery was fast, substantially extracellular Cl- (ClO-) and DIDS inhibitable in parietal cells but slow and ClO- -independent in chief and surface cells. These results suggest that during intracellular acidification the Na+-H+ exchanger is the major pH regulator in these three gastric cell types even in the presence of HCO3-. During alkalinization the Cl- -HCO3-(OH-) exchanger is the predominant pH recovery mechanism in parietal, but not in chief and surface cells. In parietal cells, this exchanger is also involved in recovery from acidification.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
256
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G466-75
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Effect of CO2 on pHi in rabbit parietal, chief, and surface cells.
pubmed:affiliation
Department of Surgery, Beth Israel Hospital, Boston, Massachusetts 02215.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't