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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-3-29
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pubmed:abstractText |
We have previously reported that egg granulomas isolated from livers of Schistosoma mansoni-infected euthymic mice in vitro elaborate a factor(s) that stimulates a variety of fibroblast responses including fibroblast proliferation and enhanced synthesis of extracellular matrix proteins. We have postulated that these factors play a role in the pathogenesis of hepatic fibrosis in schistosomiasis mansoni. Serum-free supernatants from egg granuloma cultures also stimulate thymocyte proliferation in an assay that defects interleukin-1 (IL-1). Thymocytes and fibroblasts are stimulated to proliferate by the same fractions of egg granuloma culture supernatant separated by gel filtration, isoelectric focusing, and ion-exchange chromatography. This suggested that granuloma-derived IL-1 is responsible for the observed fibroblast stimulation. Here we report that the ability of granuloma culture supernatants to stimulate the IL-1-sensitive D10.G4.1 cells but not fibroblasts is removed by treatment with immobilized anti-IL-1 antibody. We also observed that dialyzed culture supernatants from egg granulomas obtained from infected congenitally athymic (nude) mice also stimulate fibroblast proliferation. Treatment with anti-IL-1 antibody did not abrogate this response. In contrast to our experience with egg granulomas isolated from euthymic mice, IL-1- and fibroblast-stimulating activity could be separated by gel filtration and isoelectric focusing. We conclude that the fibroblast growth-stimulating activities elaborated by egg granulomas from S. mansoni-infected euthymic and athymic mice may be different but both appear to be distinct from IL-1.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-2582266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-2949327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-2960773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-303056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-307029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-3100642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-310861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-3155791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-316981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-3326134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-3491144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-3495799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-3497982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-4971842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6213718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6235287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6268716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6325504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6332149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6369481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6460819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-648016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6557014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6608569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-6970264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-7119116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-80435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-825607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-932208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2492969-961824
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
679-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2492969-Animals,
pubmed-meshheading:2492969-Biological Assay,
pubmed-meshheading:2492969-Chromatography, Gel,
pubmed-meshheading:2492969-Fibroblasts,
pubmed-meshheading:2492969-Granuloma,
pubmed-meshheading:2492969-Growth Substances,
pubmed-meshheading:2492969-Immunologic Techniques,
pubmed-meshheading:2492969-Interleukin-1,
pubmed-meshheading:2492969-Interleukin-2,
pubmed-meshheading:2492969-Interleukin-4,
pubmed-meshheading:2492969-Interleukins,
pubmed-meshheading:2492969-Isoelectric Point,
pubmed-meshheading:2492969-Mice,
pubmed-meshheading:2492969-Mice, Nude,
pubmed-meshheading:2492969-Schistosomiasis mansoni
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pubmed:year |
1989
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pubmed:articleTitle |
Schistosomal egg granuloma-derived fibroblast-stimulating factor is apparently distinct from interleukin-1.
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pubmed:affiliation |
Department of Medicine, New England Medical Center Hospitals, Boston, Massachusetts.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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