Linked Life Data

2483447

Source:http://linkedlifedata.com/resource/pubmed/id/2483447

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-4-13
pubmed:abstractText
Alterations in cytosolic metabolites and [Mg2+]i were monitored using 31P magnetic resonance spectroscopy during inotropic stimulation of isolated rat heart [10 nM isoproterenol, 0.6 microM isobutyl-1-methylxanthine (IBMX), 5 microM ouabain]. All drugs significantly elevated contractile function (rate-pressure product) and MVO2 by approximately 100-150% (P less than 0.001), decreased cytosolic [creatine phosphate] ([CrP]) and [ATP] (approximately 65 and 80% of control values, respectively) (P less than 0.001), increased [Pi] to more than 180% of pretreatment values, and decreased [H+] by less than 15% (P less than 0.05). A significant relative shift in the alpha-P and beta-P resonances of ATP (P less than 0.01) occurred with inotropic stimulation. [Mg2+]i calculated on the basis of these shifts was found to be 0.78 +/- 0.1 mM in control hearts, and increased to maxima of 1.9 +/- 0.2, 2.0 +/- 0.2, and 2.9 +/- 0.2 mM during infusion of isoproterenol, IBMX, and ouabain, respectively. Changes in [Mg2+]i correlate with cytosolic [ATP] + [CrP] in all hearts (r = 0.89, 0.91, and 0.88 in isoproterenol-, IBMX-, and ouabain-treated hearts, respectively). The significantly higher [Mg2+]i with ouabain infusion (P less than 0.01) at similar workloads and [ATP] + [CrP] supports the proposal that a ouabain-inhibited Mg2+ pump exists in the plasma membrane. The data support acute changes in [Mg2+]i during alterations in inotropic state that may be important in modulating metabolic and contractile function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0740-3194
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
328-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Effect of inotropic stimulation on cytosolic Mg2+ in isolated rat heart: a 31P magnetic resonance study.
pubmed:affiliation
Division of Science and Technology, Griffith University, Queensland, Australia.
pubmed:publicationType
Journal Article, In Vitro