|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1990-3-14
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|
pubmed:abstractText |
The porin of Thiobacillus versutus IFO 14567 was isolated by extraction of cell-envelopes with sodium dodecyl sulfate. It exhibited strong porin-activity after reconstitution into artificial lipid bilayer membranes. The diameter of the pore was determined as 1.6 nm, with a weak selectivity for cations being observed. The porin migrated as a single band (Mr 35 kDa) on SDS-polyacrylamide gel-electrophoresis after heating (100 degrees C, 5 min). The porin oligomer was not sensitive towards EDTA. Analytical ultracentrifugation studies demonstrated the native oligomer to be a trimer.
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|
pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0378-1097
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
53
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
319-22
|
|
pubmed:dateRevised |
2006-11-15
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|
pubmed:meshHeading |
|
|
pubmed:year |
1989
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|
pubmed:articleTitle |
Porin from Thiobacillus versutus.
|
|
pubmed:affiliation |
Institut für Biologie II, Mikrobiologie, Albert Ludwigs Universität, Freiburg, F.R.G.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
