Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-7-11
pubmed:databankReference
pubmed:abstractText
A genetic system that allows the cloning of a peptide-coding sequence in the Escherichia coli K88ac and K88ad pilin genes and their expression as recombinant pili has been constructed. Two insertion vectors were created by subcloning the pilin genes in a pBR322 plasmid and replacing the coding sequence of two nonconserved clusters by a linker. The K88ac helper genes were subcloned in the compatible pACYC184 plasmid, and expression of pili by bacteria carrying both plasmids occurred by complementation. Two peptide-coding sequences of the influenza hemagglutinin were cloned in both insertion vectors, and recombinant pilins were shown to be assembled in pili. One recombinant pilus was shown to elicit antibodies against the synthetic peptide in immunized rats. The somatostatin-coding sequence was cloned in both vectors and led in one case to detectable pilus production. The fused somatostatin was shown to be recognized by specific monoclonal and polyclonal antibodies.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-13690195, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-149110, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2412961, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2467165, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-265521, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2862689, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2864360, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2865086, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2870470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2879831, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-2907768, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-3078832, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-412251, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-4868368, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-4960185, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-6153930, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-6176330, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-6184717, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-6309738, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-6310323, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-6371814, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-6751939, http://linkedlifedata.com/resource/pubmed/commentcorrection/2471451-7017708
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
984-93
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:2471451-Animals, pubmed-meshheading:2471451-Antibodies, Bacterial, pubmed-meshheading:2471451-Antigens, Bacterial, pubmed-meshheading:2471451-Antigens, Surface, pubmed-meshheading:2471451-Bacterial Outer Membrane Proteins, pubmed-meshheading:2471451-Base Sequence, pubmed-meshheading:2471451-Cloning, Molecular, pubmed-meshheading:2471451-DNA, Bacterial, pubmed-meshheading:2471451-Epitopes, pubmed-meshheading:2471451-Escherichia coli, pubmed-meshheading:2471451-Escherichia coli Proteins, pubmed-meshheading:2471451-Fimbriae, Bacterial, pubmed-meshheading:2471451-Fimbriae Proteins, pubmed-meshheading:2471451-Gene Expression Regulation, pubmed-meshheading:2471451-Genetic Complementation Test, pubmed-meshheading:2471451-Immunoassay, pubmed-meshheading:2471451-Immunoblotting, pubmed-meshheading:2471451-Immunohistochemistry, pubmed-meshheading:2471451-Microscopy, Electron, pubmed-meshheading:2471451-Molecular Sequence Data, pubmed-meshheading:2471451-Peptides, pubmed-meshheading:2471451-Plasmids, pubmed-meshheading:2471451-Rats, pubmed-meshheading:2471451-Rats, Inbred Strains, pubmed-meshheading:2471451-Restriction Mapping
pubmed:year
1989
pubmed:articleTitle
Cloning of DNA sequences encoding foreign peptides and their expression in the K88 pili.
pubmed:affiliation
Central Laboratory, Solvay et Cie, Brussels, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't