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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1989-5-5
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pubmed:abstractText |
Human immunodeficiency virus type 2 (HIV-2)-related viruses were isolated from a Gambian dying of exclusively neurological disease (HIV-2D194) and from an asymptomatic Ghanian (HIV-2D205). Both strains exhibited properties of HIV-1 biological subtype c: they grew slowly and induced few or no syncytia but eventually produced high levels of particle-associated reverse transcriptase in cultures of fresh peripheral blood lymphocytes, and they established stable infection of T-lymphoma (HUT-78) and monocytic (U937) cell lines. Each produced even higher levels of reverse transcriptase when fresh human monocytes/macrophages were used as target cells. The viruses were molecularly cloned after a single passage in culture, in order to minimize in vitro selection of subtypes present in vivo. Restriction-site analysis showed heterogeneity within each isolate. Nucleotide sequence analysis of a portion of the HIV-2D194 genome revealed that it is a member of the prototypic HIV-2 family, displaying 13% divergence versus HIV-2ROD and HIV-2NIHZ, as compared to 9% divergence between HIV-2ROD and HIV-2NIHZ. In contrast, HIV-2D205 is the most highly divergent HIV-2 strain yet described: it is equidistant in relation between the known HIV-2 strains and the simian immunodeficiency virus isolates from rhesus macaque monkeys (23-25% divergence).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2467304-2425430,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
2383-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2467304-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:2467304-Cells, Cultured,
pubmed-meshheading:2467304-Cloning, Molecular,
pubmed-meshheading:2467304-DNA, Viral,
pubmed-meshheading:2467304-Gambia,
pubmed-meshheading:2467304-Genes, Viral,
pubmed-meshheading:2467304-HIV-2,
pubmed-meshheading:2467304-Humans,
pubmed-meshheading:2467304-Lymphocytes,
pubmed-meshheading:2467304-Macrophages,
pubmed-meshheading:2467304-Nervous System Diseases,
pubmed-meshheading:2467304-RNA-Directed DNA Polymerase,
pubmed-meshheading:2467304-Restriction Mapping,
pubmed-meshheading:2467304-Virus Replication
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pubmed:year |
1989
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pubmed:articleTitle |
Molecular cloning of two west African human immunodeficiency virus type 2 isolates that replicate well in macrophages: a Gambian isolate, from a patient with neurologic acquired immunodeficiency syndrome, and a highly divergent Ghanian isolate.
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pubmed:affiliation |
Chemotherapeutisches Forschungsinstitut, Frankfurt, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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