Linked Life Data

2464079

Source:http://linkedlifedata.com/resource/pubmed/id/2464079

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-4
pubmed:dateCreated
1989-3-8
pubmed:abstractText
We have studied gene expression of neuroglial cell markers in the myelin-deficient (md) rat brain during postnatal development. Northern blots and slot blots of poly(A)+ RNA from developing brain were sequentially probed with cDNAs specific for the oligodendrocyte markers glycerol phosphate dehydrogenase (GPDH), myelin basic protein (MBP), and proteolipid protein (PLP), for the neuronal marker glutamic acid decarboxylase (GAD), and for the astrocyte markers glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS). GPDH mRNA levels were also examined in two peripheral tissues, liver, and skeletal muscle (hindlimb). Despite a lack of CNS myelin in the md mutant, transcripts of all oligodendroglial markers were detectable except the 1.6-kb PLP message. Brain GPDH mRNA levels were initially equivalent in md and unaffected littermates at postnatal day 15 (PI5), but the mutants failed to display the normal developmental increase in gene expression. By P25, GPDH mRNA expression in md rat brain was approximately 20% of control levels. GPDH mRNA expression in peripheral tissues was less affected than in brain and was lower in md mutants only at the later developmental ages. Expressions of GAD, GFAP, and GS mRNAs in developing md rat brain were not altered. The mRNA levels of the two myelin markers, MBP and PLP, were severely impaired in md rat brain during the entire myelinating period and represented less than 10% of control mRNA levels at P25. The most important observation was that the large PLP transcript (3.2 kb) was slightly shorter in size in md rat brain as compared to normals.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
268-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Developmental expression of neural cell type-specific mRNA markers in the myelin-deficient mutant rat brain: inhibition of oligodendrocyte differentiation.
pubmed:affiliation
Department of Anatomy, UCLA School of Medicine.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.