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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1988-12-6
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pubmed:abstractText |
A diagnostic test for the detection of Streptococcus pneumoniae meningitis was developed using monoclonal antibodies (MAbs) to phosphocholine (PC) and non-PC determinants of pneumococcal teichoic acids. These MAbs do not recognize other bacteria that commonly cause meningitis. By using a dot blot assay, these MAbs were compared with a polyvalent pneumococcal capsular omniserum and an antiserum made to whole cells for their ability to detect pneumococci in infected spinal fluids. An immunoglobulin M (IgM) anti-PC antibody gave a positive reaction with 16 of 22 (73%) pneumococcal culture-positive spinal fluids. One false-positive result out of 45 pneumococcal culture-negative spinal fluids was also observed. D3114/63, an IgM MAb to non-PC determinants of teichoic acids, detected 15 of 22 of the pneumococcal culture-positive spinal fluids with one false-positive result. IgG2b and IgG3 anti-PC MAbs were less efficient than the IgM anti-PC MAb at detecting pneumococci in spinal fluids. Like the IgM anti-PC MAb, omniserum detected 73% of the culture-positive pneumococcal spinal fluids, with one false-positive result. The use of anti-PC or D3114/63 MAbs instead of a pooled serum such as omniserum has several advantages: (i) use of a single cross-reactive antibody rather than 83 pooled antibodies; (ii) possibility of a higher concentration of reactive antibody, which may increase the sensitivity of the test; (iii) a standardized antibody preparation; (iv) ease of preparation of the antibody; and (v) less expense.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-13301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-2462151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-31678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-3733218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-3889046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-3952010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-3958504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-4118732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-4381230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-6336560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-6418756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-6436296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-67124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-7020043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-7231520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2460493-7299349
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1635-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2460493-Antibodies, Monoclonal,
pubmed-meshheading:2460493-Antibody Specificity,
pubmed-meshheading:2460493-Antigens, Bacterial,
pubmed-meshheading:2460493-Cell Wall,
pubmed-meshheading:2460493-Cross Reactions,
pubmed-meshheading:2460493-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2460493-Epitopes,
pubmed-meshheading:2460493-False Positive Reactions,
pubmed-meshheading:2460493-Humans,
pubmed-meshheading:2460493-Immunoblotting,
pubmed-meshheading:2460493-Immunoglobulin G,
pubmed-meshheading:2460493-Immunoglobulin M,
pubmed-meshheading:2460493-Meningitis, Pneumococcal,
pubmed-meshheading:2460493-Phosphorylcholine,
pubmed-meshheading:2460493-Predictive Value of Tests,
pubmed-meshheading:2460493-Streptococcus pneumoniae,
pubmed-meshheading:2460493-Teichoic Acids
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pubmed:year |
1988
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pubmed:articleTitle |
Cross-reactive monoclonal antibodies for diagnosis of pneumococcal meningitis.
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pubmed:affiliation |
Department of Microbiology, University of Alabama, Birmingham 35294.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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