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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-11-23
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pubmed:abstractText |
A version of Mallory's phloxine-methylene blue-azure II technique suitable for large epoxy sections is described. Phloxine B (C.I. 45410) and a yellow-green interference filter (546-548 nm transmission) combine to give high contrast monochrome images. By comparing light micrographs of lung parenchyma entirely unstained or stained only with phloxine B against electron micrographs of the same material, it is seen that phloxine B emphasizes essentially only elastin and collagen fiber bundles. The technique has produced images useful for investigating lung parenchyma architecture and micromechanics.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0038-9153
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
165-70
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2459814-Animals,
pubmed-meshheading:2459814-Azure Stains,
pubmed-meshheading:2459814-Collagen,
pubmed-meshheading:2459814-Dogs,
pubmed-meshheading:2459814-Elastin,
pubmed-meshheading:2459814-Eosine I Bluish,
pubmed-meshheading:2459814-Epoxy Resins,
pubmed-meshheading:2459814-Lung,
pubmed-meshheading:2459814-Methylene Blue,
pubmed-meshheading:2459814-Microtomy,
pubmed-meshheading:2459814-Staining and Labeling
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pubmed:year |
1988
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pubmed:articleTitle |
Mallory's phloxine B-methylene blue-azure II stain emphasizes elastin and collagen bundles in epoxy embedded lung.
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pubmed:affiliation |
Division of Pulmonary Medicine, Memorial Hospital of Rhode Island, Providence.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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