Linked Life Data

2457585

Source:http://linkedlifedata.com/resource/pubmed/id/2457585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1988-9-28
pubmed:abstractText
Endonuclease activity which specifically cleaves baseless (apurinic/apyrimidinic (AP] sites in supercoiled DNA has been purified from mitochondria of the mouse plasmacytoma cell line, MPC-11. Two variant forms separate upon purification; these have small but reproducible differences in catalytic and chromatographic properties, but similar physical properties. Both have a sedimentation coefficient of 4.0, corresponding to a molecular weight of 61,000 (assuming a globular configuration) and a peptide molecular weight of about 65,000 as determined by immunoblot analysis with antiserum raised against the major AP endonuclease from HeLa cells. Thus mitochondrial AP endonuclease appears to be a monomer of about 65 kDa, making it distinguishable from the major AP endonuclease of MPC-11 cells which, like those of other mammalian cells, appears to be a monomer of about 41 kDa. A possible 82-kDa precursor form was also detected by immunoblot analysis of a crude mitochondrial fraction. Mitochondrial AP endonuclease activity is greatly stimulated by divalent cations, has a pH optimum between 6.5 and 8.5, and cleaves the AP site by a class II mechanism to generate a 3'-OH nucleotide residue. These properties resemble those of the major mammalian AP endonucleases but, unlike those enzymes, mitochondrial AP endonuclease activity is neither inhibited by adenine or NAD+ nor stimulated by Triton X-100. Since the mitochondrial activity generates active primer termini for DNA synthesis, it could function in base excision DNA repair; alternatively, it might have a role in eliminating damaged mitochondrial genomes from the gene pool.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12532-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2457585-Apurinic Acid, pubmed-meshheading:2457585-Cations, Divalent, pubmed-meshheading:2457585-Centrifugation, Density Gradient, pubmed-meshheading:2457585-DNA, pubmed-meshheading:2457585-DNA, Superhelical, pubmed-meshheading:2457585-DNA-(Apurinic or Apyrimidinic Site) Lyase, pubmed-meshheading:2457585-Deoxyribonuclease IV (Phage T4-Induced), pubmed-meshheading:2457585-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:2457585-Endodeoxyribonucleases, pubmed-meshheading:2457585-Hydrogen-Ion Concentration, pubmed-meshheading:2457585-Immunosorbent Techniques, pubmed-meshheading:2457585-Mitochondria, pubmed-meshheading:2457585-Molecular Weight, pubmed-meshheading:2457585-Plasmacytoma, pubmed-meshheading:2457585-Polynucleotides, pubmed-meshheading:2457585-Substrate Specificity, pubmed-meshheading:2457585-Tumor Cells, Cultured
pubmed:year
1988
pubmed:articleTitle
Mitochondrial endonuclease activities specific for apurinic/apyrimidinic sites in DNA from mouse cells.
pubmed:affiliation
Department of Biochemistry, University of California, Berkeley 94720.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't