rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1988-6-21
|
pubmed:abstractText |
The 105 kDa antigen of Plasmodium chabaudi has many characteristics of the P. falciparum Pf 155 (RESA) molecule. A clone (pPC105e) from a P. chabaudi genomic library was isolated using immune screening with a 105 kDa antigen specific monoclonal antibody (B7E10). Southern, Northern and Western blotting analyses provide evidence for a lack of variability at the protein and DNA levels. A subclone of the insert in the expression vector pEX2, synthesises a fusion peptide which contains the epitope recognized by B7E10. Sequences homologous to the insert were detected in the genome of three other rodent and two primate malarias.
|
pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0166-6851
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
28
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
153-61
|
pubmed:dateRevised |
2010-8-25
|
pubmed:meshHeading |
pubmed-meshheading:2452981-Animals,
pubmed-meshheading:2452981-Antigens, Protozoan,
pubmed-meshheading:2452981-Cloning, Molecular,
pubmed-meshheading:2452981-DNA,
pubmed-meshheading:2452981-DNA Restriction Enzymes,
pubmed-meshheading:2452981-Deoxyribonuclease EcoRI,
pubmed-meshheading:2452981-Electrophoresis, Agar Gel,
pubmed-meshheading:2452981-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2452981-Epitopes,
pubmed-meshheading:2452981-Immunoassay,
pubmed-meshheading:2452981-Nucleic Acid Hybridization,
pubmed-meshheading:2452981-Plasmodium,
pubmed-meshheading:2452981-RNA,
pubmed-meshheading:2452981-Recombinant Fusion Proteins
|
pubmed:year |
1988
|
pubmed:articleTitle |
Cloning of genomic fragment from Plasmodium chabaudi expressing a 105 kilodalton antigen epitope.
|
pubmed:affiliation |
Division of Parasitology, National Institute for Medical Research, London, U.K.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|