Linked Life Data

2450128

Source:http://linkedlifedata.com/resource/pubmed/id/2450128

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1988-4-20
pubmed:abstractText
The V beta 8-specific mAb F23.1 and KJ16 were used as fluorescent stains to test for TCR expression on the surface of subpopulations of early, CD4-CD8- (L3T4-Ly-2-) thymocytes from adult CBA mice. A surprisingly high proportion (27%) of Ly-2-L3T4- thymocytes were strongly F23.1 and KJ16 positive. No positive cells were detected among Ly-2-L3T4- thymocytes from V beta 8-negative SJL mice. In contrast to the adult thymus, Ly-2-L3T4- cells from embryonic CBA thymus lacked F23.1-positive cells. Subsets of adult CBA Ly-2-L3T4- thymocytes were separated to determine which expressed V beta 8. The major subset, Ly-1 low B2A2-M1/69+Thy-1+Pgp-1-, representing a phenotype similar to embryonic Ly-2-L3T4- thymocytes and the phenotype commonly isolated from adult thymocytes as Ly-1 "dull," lacked cells strongly positive for F23.1. In contrast, a series of subsets of adult CBA Ly-2-L3T4- thymocytes which were B2A2-M1/69- and Pgp-1+ all included strongly F23.1-positive cells. A minor subset, negative for most markers except Pgp-1 and presumed on the basis of this phenotype and some reconstitution studies to include the earliest intrathymic precursors, contained 28% F23.1-positive cells. However, no F.23.1-positive cells were detected in equivalent "prethymic" populations from bone marrow or from athymic mouse spleen. The subsets of Ly-2-L3T4- thymocytes which were Ly-1 high, B2A2-M1/69-, and Pgp-1+ all contained about 70% F23.1-positive cells, indicating a V beta 8 usage much higher than the mature T cell average. These results indicate that a series of distinct developmental events have occurred within these CD4-CD8- thymocytes previously considered as a single group of early precursor cells, and that some aspects of repertoire selection may be occurring amongst thymocytes which lack CD4 or CD8.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
140
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1470-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:2450128-Aging, pubmed-meshheading:2450128-Animals, pubmed-meshheading:2450128-Antibodies, Monoclonal, pubmed-meshheading:2450128-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:2450128-Antigens, Ly, pubmed-meshheading:2450128-Antigens, Surface, pubmed-meshheading:2450128-Cell Line, pubmed-meshheading:2450128-Embryo, Mammalian, pubmed-meshheading:2450128-Fluorescent Dyes, pubmed-meshheading:2450128-Hematopoietic Stem Cells, pubmed-meshheading:2450128-Male, pubmed-meshheading:2450128-Membrane Glycoproteins, pubmed-meshheading:2450128-Mice, pubmed-meshheading:2450128-Mice, Inbred CBA, pubmed-meshheading:2450128-Mice, Nude, pubmed-meshheading:2450128-Phenotype, pubmed-meshheading:2450128-Receptors, Antigen, T-Cell, pubmed-meshheading:2450128-Receptors, Lymphocyte Homing, pubmed-meshheading:2450128-Staining and Labeling, pubmed-meshheading:2450128-T-Lymphocytes
pubmed:year
1988
pubmed:articleTitle
T cell antigen receptor expression by subsets of Ly-2-L3T4- (CD8-CD4-) thymocytes.
pubmed:affiliation
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't