Linked Life Data

2448468

Source:http://linkedlifedata.com/resource/pubmed/id/2448468

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-3-10
pubmed:abstractText
The effect of the carboxyl group activating reagent N-ethoxy-carbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) on the gating current of the frog node of Ranvier was investigated. A 10-min treatment with 2 mM EEDQ (in the presence or absence of 10 mM ethylenediamine) irreversibly reduced the slope of the on charge-voltage relation Qon(E), shifted its midpoint potential Emid in the positive direction and reduced the maximum charge Qon max measured with strong depolarizing pulses. In six experiments, 2 mM EEDQ + 10 mM ethylenediamine increased the factor k (a reciprocal measure of the slope of the Qon(E) curve) from 16 to 22 mV. In five experiments, 2 mM EEDQ alone increased k from 16 to 23 mV. In a single experiment, 5 mM EEDQ + 10 mM ethylene diamine increased k from 17 to 31 mV. The reduction in slope suggests that EEDQ decreases the valence of the gating particles or reduces the fraction of the membrane field that they traverse. In addition, EEDQ (which inhibits inactivation of the sodium current, see M. Rack and K.H. Woll, J. Membrane Biol. 82:41-48, 1984) caused a small increase of the off charge Qoff, and a marked increase of the Qoff/Qon ratio, i.e. inhibited charge immobilization. Since the effects of EEDQ occurred regardless of the presence or absence of ethylenediamine, they are probably due to crosslinking reactions. The effects of EEDQ were compared with those of the water-soluble carbodiimide EDC. Treatment with 10 or 50 mM EDC (plus 10 or 50 mM ethylenediamine) caused a smaller increase of k than treatment with 2 mM EEDQ but reduced Qon max by the same amount.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Effects of reagents modifying carboxyl groups on the gating current of the myelinated nerve fiber.
pubmed:affiliation
I. Physiologisches Institut, Universität des Saarlandes, Homburg/Saar, Federal Republic of Germany.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't