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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1987-2-11
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pubmed:abstractText |
Two types of divalent cation conducting channels from rabbit skeletal muscle sarcoplasmic reticulum (SR) were incorporated into planar lipid bilayers. A high conductance (100 pS in 53 mM trans Ca2+) Ca2+ channel was incorporated from heavy density SR fractions. The 100-pS channel was activated by adenine nucleotides and Ca2+ and inhibited by Mg2+ and ruthenium red. A 10-pS calcium and barium conducting channel could be incorporated into planar lipid bilayers from light, intermediate, and heavy density SR vesicles. 10-pS channel activity in bilayers was not dependent on cis Ca2+ and was only weakly dependent on adenine nucleotides. Ruthenium red at concentrations up to 1 mM had no effect and Mg2+ was only marginally effective in inhibiting macroscopic Ba2+ currents from this channel. Calcium releasing activity in intermediate and heavy density SR fractions was assayed according to a rapid quench protocol and compared with the results obtained in the bilayer. Results from this comparison indicate that the 10-pS channel is probably not involved in rapid Ca2+- and adenine nucleotide-induced Ca2+ release from isolated SR vesicles.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-13441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-159829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-2410794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-2410798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-2994073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-3754147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-489590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6090649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6147356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6233277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6279756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6284941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6309780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6312285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6330279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6346892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6490650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6654845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6698971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431726-6796647
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
921-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1986
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pubmed:articleTitle |
Single-channel calcium and barium currents of large and small conductance from sarcoplasmic reticulum.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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