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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0185117,
umls-concept:C0301812,
umls-concept:C0442040,
umls-concept:C0521115,
umls-concept:C0677626,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1987-1-14
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pubmed:databankReference |
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pubmed:abstractText |
The tissue-specific expression of two types of mouse amylase genes does not overlap in vivo; the Amy-1 locus is transcribed in the parotid gland and the liver, while expression of Amy-2 is limited to the pancreas. We identified a mouse hepatoma cell line, Hepa 1-6, in which both amylase genes can be simultaneously expressed. Amy-1 is constitutively active in these cells and is inducible by dexamethasone at the level of mRNA. We demonstrated that the liver-specific promoter of Amy-1 is utilized by the dexamethasone-treated hepatoma cells, and that glucocorticoid consensus sequences are present upstream of this promoter. Amy-2 is not detectable constitutively, but can be activated if the cells are cultured in serum-free medium containing dexamethasone. Expression of Amy-2 in a nonpancreatic cell type has not previously been observed. We speculate that induction of Amy-1 and activation of Amy-2 may involve different regulatory mechanisms. Hepa 1-6 cells provide an experimental system for molecular analysis of these events.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-179580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-200463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-226517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-2410924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-2579957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-264117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-2989529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-3872721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-4831907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6091192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6102619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6157477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6161122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6162108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6162570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6163812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6176715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6180955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6190572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6205392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6296163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6323998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-6754087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-7204370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-721808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2431276-94023
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
969-75
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2431276-Amylases,
pubmed-meshheading:2431276-Animals,
pubmed-meshheading:2431276-Base Sequence,
pubmed-meshheading:2431276-Cell Line,
pubmed-meshheading:2431276-Dexamethasone,
pubmed-meshheading:2431276-Enzyme Induction,
pubmed-meshheading:2431276-Genes,
pubmed-meshheading:2431276-Isoenzymes,
pubmed-meshheading:2431276-Liver,
pubmed-meshheading:2431276-Liver Neoplasms, Experimental,
pubmed-meshheading:2431276-Mice,
pubmed-meshheading:2431276-Mice, Inbred C57BL,
pubmed-meshheading:2431276-Pancreas,
pubmed-meshheading:2431276-Parotid Gland,
pubmed-meshheading:2431276-Promoter Regions, Genetic,
pubmed-meshheading:2431276-Saliva,
pubmed-meshheading:2431276-Transcription, Genetic
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pubmed:year |
1986
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pubmed:articleTitle |
Simultaneous expression of salivary and pancreatic amylase genes in cultured mouse hepatoma cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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