Linked Life Data

2416849

Source:http://linkedlifedata.com/resource/pubmed/id/2416849

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-2-13
pubmed:abstractText
This report describes an investigation of the abilities of different immunoassays to detect differences in affinities between related antigens for particular monoclonal antibodies. Nine different monoclonal antibodies were used and 6 strains of influenza virus represented closely related antigens. Parameters defining assay sensitivity were estimated experimentally for each antibody in 4 different immunoassays. Predicted failures of particular assays to detect differences in antigen affinities, based on these parameters, were demonstrated. One assay method failed to detect heteroclitic activity of 1 antibody which was clearly evident in the other 3 assays. As well as supporting theoretical models of assay sensitivity derived in the preceding paper the experiments demonstrated a significant effect of antibody subclass.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
309-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Practical considerations of the ability of monoclonal antibodies to detect antigenic differences between closely related variants.
pubmed:publicationType
Journal Article, Comparative Study