Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
18
|
pubmed:dateCreated |
1986-2-6
|
pubmed:abstractText |
Deoxy-5-azacytidine 5'-triphosphate was synthesized and used as a substrate for the enzymatic synthesis of the polynucleotide poly[d(G-z5C)]. Whereas the triphosphate decomposes in solution, the azacytosine analogue incorporated into DNA is stable under conditions preserving the double-helical structure. Poly[d(G-z5C)] undergoes the transition to the left-handed Z conformation at salt (NaCl and MgCl2) concentrations approximately 30% higher than those required for unsubstituted poly[d(G-C)]. However, the incorporation of azacytidine potentiates the formation at room temperature of the Z helix stabilized by the transition metal Mn2+; in the case of poly[d(G-C)], a heating step is required. The spectral properties of the two polymers in the B and Z forms are similar. Both left-handed forms are recognized by anti-Z DNA immunoglobulins, indicating that the DNAs bear common antigenic features. Poly[d(G-z5C)] is not a substrate for the DNA cytosine 5-methyltransferase from human placenta. It is a potent inhibitor of the enzyme when tested in a competitive binding assay. These results are compatible with a very strong, possibly covalent, mode of interaction between methyltransferases and DNA containing 5-azacytosine.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-aza-2'-deoxycytidine-5'-triphospha...,
http://linkedlifedata.com/resource/pubmed/chemical/Azacitidine,
http://linkedlifedata.com/resource/pubmed/chemical/Cytidine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/DNA (Cytosine-5-)-Methyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Polydeoxyribonucleotides
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0006-2960
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
27
|
pubmed:volume |
24
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
4806-14
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:2416336-Azacitidine,
pubmed-meshheading:2416336-Cytidine Triphosphate,
pubmed-meshheading:2416336-DNA (Cytosine-5-)-Methyltransferase,
pubmed-meshheading:2416336-Female,
pubmed-meshheading:2416336-Humans,
pubmed-meshheading:2416336-Indicators and Reagents,
pubmed-meshheading:2416336-Kinetics,
pubmed-meshheading:2416336-Nucleic Acid Conformation,
pubmed-meshheading:2416336-Placenta,
pubmed-meshheading:2416336-Polydeoxyribonucleotides,
pubmed-meshheading:2416336-Pregnancy,
pubmed-meshheading:2416336-Spectrophotometry, Ultraviolet
|
pubmed:year |
1985
|
pubmed:articleTitle |
Synthesis and characterization of poly[d(G-z5C)]. B-Z transition and inhibition of DNA methylase.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|