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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1986-1-6
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pubmed:abstractText |
We have observed a modification of the cellular protein kinase pp60c-src, elicited in murine 3T3 fibroblasts by platelet-derived growth factor (PDGF). The modification occurred rapidly after addition of PDGF to the culture medium and was first detected as a reduction in the electrophoretic mobility of a portion of the pp60c-src molecules. A similarly modified form of the viral homologue pp60v-src occurs in vivo in the absence of stimulation by PDGF. The occurrence of modified forms of both pp60c-src and pp60v-src was associated with a novel phosphorylation at tyrosine in the amino-terminal domains of the proteins. The time-course and dose-response for this modification of pp60c-src paralleled PDGF-induced increases in phosphorylation of pp36, a major cellular substrate for several tyrosine-specific protein kinases. In parallel experiments, treatment of cells with PDGF increased the kinase activity of pp60c-src in an immunocomplex assay. These results suggest pp60c-src may play a role in the mitogenic response to PDGF.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-167110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-176594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-221907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-226956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-228282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-2987699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-312292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-3927285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-5277067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6090941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6091900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6091918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6095053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6100962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6173766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6193631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6204986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6246487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6256641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6267776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6270659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6292480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6294529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6311433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6314123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6326105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6341813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6402662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6426802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6437865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6686688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6795201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-6852247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2415973-7263691
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7845-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2415973-Amino Acid Sequence,
pubmed-meshheading:2415973-Animals,
pubmed-meshheading:2415973-Cells, Cultured,
pubmed-meshheading:2415973-Enzyme Activation,
pubmed-meshheading:2415973-Mice,
pubmed-meshheading:2415973-Mitosis,
pubmed-meshheading:2415973-Phosphorylation,
pubmed-meshheading:2415973-Phosphotyrosine,
pubmed-meshheading:2415973-Platelet-Derived Growth Factor,
pubmed-meshheading:2415973-Proto-Oncogene Proteins,
pubmed-meshheading:2415973-Proto-Oncogene Proteins pp60(c-src),
pubmed-meshheading:2415973-Tyrosine
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pubmed:year |
1985
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pubmed:articleTitle |
The product of the protooncogene c-src is modified during the cellular response to platelet-derived growth factor.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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