Linked Life Data

2407272

Source:http://linkedlifedata.com/resource/pubmed/id/2407272

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-4-3
pubmed:abstractText
The understanding of biologic and pathophysiologic processes in lung is aided by a technique for ascertaining the transcriptional phenotype of small numbers of cells examined in vivo. The mRNA phenotyping procedure consists of scaled-down methods for isolating mRNA from small numbers of cells, followed by reverse transcription of the RNA and specifically primed amplification of the cDNA by the polymerase chain reaction. We show an example of the use of the technique in a study of expression of platelet-derived growth factor and other growth factor genes in macrophages isolated from wound cylinders implanted in mice. With this technique, the transcriptional phenotype of purified normal lung epithelial and mesenchymal cells, macrophages, or cells obtained from lavaged lungs or punch biopsy specimens can be examined as a rapid first step in understanding molecular processes in the lung.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-1549
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3-10
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
mRNA phenotyping for studying gene expression in small numbers of cells: platelet-derived growth factor and other growth factors in wound-derived macrophages.
pubmed:affiliation
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.