Linked Life Data

2396503

Source:http://linkedlifedata.com/resource/pubmed/id/2396503

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1990-10-10
pubmed:abstractText
Elevated levels of bilirubin and other tetrapyrroles are common to a number of chronic hematologic and liver diseases that can result in growth failure. This report establishes a cellular model system for the study of these endogenous growth inhibitors. Primary chondrocyte cultures were prepared from embryonic chick cartilage; cells were incubated (0.3 X 10(5) cells per plate) in tissue culture medium containing 10% fetal bovine serum (FBS) with or without added bilirubin, 0.01-0.10 mM. Bilirubin caused profound, dose-dependent inhibition of chondrocyte proliferation: after 7 days, control incubations contained 11.45 +/- 2.0 X 10(5) cells per plate versus 4.92 +/- 0.55 X 10(5) cells per plate for wells with added 0.01 mM bilirubin and 1.76 +/- 0.30 X 10(5) for cultures with added 0.05 mM bilirubin. In chondrocytes cultured for 3 days, the addition of 0.05 mM bilirubin was associated with inhibition of [3H]thymidine incorporation into DNA (47 +/- 5% of control), [3H]uridine incorporation into RNA (11 +/- 0.05% of control), and [14C]leucine incorporation into proteins (16 +/- 1% of control). The inhibition of chondrocyte proliferation induced by a range of bilirubin concentrations (0.01-0.10 mM) was in no way attenuated by the addition of physiologic concentrations of albumin (4 g/dl). After 3 days in media containing bilirubin or heme (at equimolar concentrations), chondrocytes were subsequently incubated in FBS alone for an additional 3 days; only partial reversal of the bilirubin (or heme)-induced inhibition was then observed.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0884-0431
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
769-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Bilirubin as an inhibitor of cartilage metabolism: effect on avian chondrocyte proliferation in cell culture.
pubmed:affiliation
Department of Medical Specialties, University of Texas M.D. Anderson Cancer Center, Houston.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.