Linked Life Data

2387757

Source:http://linkedlifedata.com/resource/pubmed/id/2387757

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-9-24
pubmed:abstractText
The influence of the membrane ionic gradient on the efflux of Fluorescein after intracellular turnover of Fluorescein diacetate was studied in HeLa cells. The kinetics of Fluorescein efflux was monitored by determining with flow cytometry the decrease in fluorescence intensity of single cells. Alterations of the Na+ and K+ gradients were induced experimentally by using ouabain, ionophores or buffers in which the ion concentration was modified. The sodium gradient was also altered by using Na(+)-co-transported amino acids. Independent evidence of these changes was obtained with a potential-sensitive indicator, 3,3'-dihexyloxacarbocyanine iodide. Conditions inducing a reduction or dissipation of the ionic gradients caused a decrease in the rate constant of Fluorescein release. In contrast, enhancement of the gradients increased the efflux rate. These results indicate that the release of Fluorescein from living cells is influenced by the membrane potential. Thus, the turnover of Fluorescein diacetate may provide a useful technique for assessing changes in membrane permeability properties related to ionic gradients.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0018-2214
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
227-33
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Intracellular turnover of fluorescein diacetate. Influence of membrane ionic gradients on fluorescein efflux.
pubmed:affiliation
Department of Animal Biology, University of Pavia, Italy.
pubmed:publicationType
Journal Article