rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-9-7
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pubmed:abstractText |
A radioimmunoassay (RIA) for antibodies to the Ro/SSA particle is described using Iodogen to radiolabel the antigenic protein moiety of Ro/SSA with 125I. RIA methods utilizing either 33% saturated ammonium sulfate (NH4)2SO4 or 3.5% polyethylene glycol to separate bound from free antigen are comparable. Either method is similar to ELISA in sensitivity and specificity. This RIA provides a fluid phase assay for the Ro/SSA-anti-Ro/SSA system not previously available.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,3,4,6-tetrachloro-3 alpha,6...,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Cytoplasmic,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/SS-A antigen,
http://linkedlifedata.com/resource/pubmed/chemical/TROVE2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Urea
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1759
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
131
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
113-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2380560-Autoantibodies,
pubmed-meshheading:2380560-Autoantigens,
pubmed-meshheading:2380560-Binding, Competitive,
pubmed-meshheading:2380560-Dose-Response Relationship, Immunologic,
pubmed-meshheading:2380560-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2380560-Humans,
pubmed-meshheading:2380560-Molecular Weight,
pubmed-meshheading:2380560-RNA, Small Cytoplasmic,
pubmed-meshheading:2380560-Radioimmunoassay,
pubmed-meshheading:2380560-Radioimmunoprecipitation Assay,
pubmed-meshheading:2380560-Ribonucleoproteins,
pubmed-meshheading:2380560-Urea
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pubmed:year |
1990
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pubmed:articleTitle |
A radioimmunoassay for antibodies to the Ro/SSA particle.
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pubmed:affiliation |
Department of Medicine, Oklahoma University College of Medicine, Oklahoma Health Sciences Center, Oklahoma City.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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