Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1990-8-21
pubmed:abstractText
The approximately 200-nucleotide-long 3'-terminal noncoding region of tobacco mosaic virus (TMV) RNA contains a tRNA-like structure and, in its immediate upstream region, three consecutive pseudoknots, each of which is composed of two double-helical segments. To elucidate the biological functions of the pseudoknot region, we constructed several deletion mutant TMV-L (a tomato strain) RNAs by using an in vitro transcription system and tested their ability to multiply in both tobacco plants and protoplasts. When deletions were introduced just downstream of the termination codon of the coat protein gene in the 5'-to-3' direction progressively, five of six double-helical segments were dispensable for viral multiplication, indicating that the pseudoknot structures are not essential for multiplication. However, extension of the deletion into the central pseudoknot region resulted in reduction in viral multiplication, accompanied by loss of development of mosaic symptoms on systemic tobacco plants. Cessation of multiplication was observed when the sequence involved in formation of double-helical segment I just upstream of the tRNA-like structure was deleted irrespective of the start point and extent of deletion. Point mutations that destabilized double-helical segment I resulted in a loss or great reduction of viral multiplication, whereas the double mutants in which the double helix was restored by additional compensating base substitutions restored multiplication to nearly the wild-type level. Thus, double-helical segment I just upstream of the tRNA-like structure is a structural feature essential for viral multiplication.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-16453568, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-16453793, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-16593669, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-17794341, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-189936, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-2452515, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3029681, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3188396, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-325560, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3323813, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3573144, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3656402, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3786131, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3839921, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-387639, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-3934645, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-4000943, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-6271278, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-6285419, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-6549346, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-6549393, http://linkedlifedata.com/resource/pubmed/commentcorrection/2370679-7214524
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3686-93
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Mutational analysis of the pseudoknot region in the 3' noncoding region of tobacco mosaic virus RNA.
pubmed:affiliation
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't