rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-6-27
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pubmed:abstractText |
A two-step zero-length crosslinking procedure for studying protein-protein complexes has been developed. One component of a complex is briefly incubated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysuccinimide resulting in the conversion of some of the protein carboxyls into succinimidyl esters. The reaction is stopped by addition of beta-mercaptoethanol and other interacting proteins are then added. Crosslinking arises from substitution of lysine epsilon-amino groups of these proteins for the succinimidyl moieties during a 1- to 2-h incubation period. The advantage of this method versus one-step zero-length crosslinking is that only one component of the complex is exposed to the crosslinker, which eliminates complications arising from the formation of crosslinks among several proteins of a multicomponent complex. Furthermore, crosslinks can be formed even in the presence of reagents, such as dithiothreitol and EDTA, that would interfere with direct crosslinking with EDC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Feb
|
pubmed:issn |
0003-2697
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
15
|
pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-5
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:2344038-Actins,
pubmed-meshheading:2344038-Animals,
pubmed-meshheading:2344038-Cross-Linking Reagents,
pubmed-meshheading:2344038-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2344038-Esters,
pubmed-meshheading:2344038-Ethyldimethylaminopropyl Carbodiimide,
pubmed-meshheading:2344038-Methods,
pubmed-meshheading:2344038-Muscles,
pubmed-meshheading:2344038-Protein Binding,
pubmed-meshheading:2344038-Proteins,
pubmed-meshheading:2344038-Rabbits,
pubmed-meshheading:2344038-Succinimides,
pubmed-meshheading:2344038-Tropomyosin,
pubmed-meshheading:2344038-Troponin
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pubmed:year |
1990
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pubmed:articleTitle |
Zero-length crosslinking procedure with the use of active esters.
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pubmed:affiliation |
Department of Muscle Research, Harvard Medical School, Massachusetts.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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