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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-7-22
|
| pubmed:abstractText |
Thymus-dependent (T) lymphocytes from (2 x 13)F1 hybrid guinea pigs immunized to ovalbumin (OVA) in complete Freund's adjuvant can be stimulated to proliferate in vitro by antigen-pulsed peritoneal exudate cells (PECs) derived from either strain 2 or strain 13 donors. In this communication, we show that the population of primed F1 T lymphocytes which can be activated by antigen-pulsed strain 2 PECs is largely independent of the population of cells that can be activated by antigen-pulsed strain 13 PECs. This was demonstrated by both positive and negative selection procedures. In the former, T lymphocytes from OVA-primed (2 x 13)F1 donors were enriched by initial culture with OVA-pulsed strain 2 or strain 13 PECs for 1 wk. Cells selected by culture with OVA-pulsed strain 2 PECs responded well to OVA-pulsed strain 2 PECs and poorly to OVA-pulsed strain 13 PECs. If positive selection had been carried out with OVA-pulsed strain 13 PECs, the selected F1 T cells responded well to OVA-pulsed 13 PECs and poorly to OVA-pulsed 2 PECs. Negative selection was achieved by short term culture with antigen-pulsed PECs and by eliminating proliferating cells by treatment with bromodeoxyuridine and light. This procedure demonstrated that the population of primed F1 T lymphocytes which are responsive to OVA or to purified protein derivative of tuberculin can be divided into subpopulations uniquely responsive to antigen on either strain 2 or strain 13 PECs. Evidence was presented to indicate that this selective responsiveness was not the result of the action of alloantigen-specific suppressor cells. The results are considered in terms of current concepts of the genetic and molecular regulation of the interaction of PECs and T lymphocytes.
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| pubmed:grant | |
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-1083875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-1084997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-1093892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-1097577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-4126770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-4541326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-4542806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-4581398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-47901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-5112203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-51901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-52677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-52686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-5481854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-59972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-62818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-63531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-776706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/233906-956724
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1007
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
145
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
618-30
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:233906-Animals,
pubmed-meshheading:233906-Antigens,
pubmed-meshheading:233906-Cells, Cultured,
pubmed-meshheading:233906-Exudates and Transudates,
pubmed-meshheading:233906-Guinea Pigs,
pubmed-meshheading:233906-Lymphocyte Activation,
pubmed-meshheading:233906-Peritoneal Cavity,
pubmed-meshheading:233906-T-Lymphocyte Subsets
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
Independent populations of primed F1 guinea pig T lymphocytes respond to antigen-pulsed parental peritoneal exudate cells.
|
| pubmed:affiliation |
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|