Linked Life Data

2338224

Source:http://linkedlifedata.com/resource/pubmed/id/2338224

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-6-21
pubmed:abstractText
Conversion of thyroxine (T4) to triiodothyronine (T3), which was demonstrated in the 6000-g supernatant fraction of kidney homogenates prepared from Rana ridibunda frogs, was influenced by substrate (0.19-1.5 microM) and homogenate (39-625 mg kidney/ml) concentration and by temperature (19.5-36.5 degrees) and duration (1 hr vs 2 hr) of incubation. Heating destroyed 5'-monodeiodinase activity. Addition of dithiothreitol (DTT) to the reaction mixture appeared to be essential to quantitate T3 production, which even increased markedly in the copresence of ethylenediaminetetraacetate (EDTA). Sensitivity of the DDT + EDTA-stimulated enzyme to propylthiouracil inhibition (25-45%) was discussed in relation with the ambient thiol concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0016-6480
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
456-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Presence of thyroxine 5'-monodeiodinase activity in kidney homogenates of the frog, Rana ridibunda.
pubmed:affiliation
Laboratory of Comparative Endocrinology, Catholic University of Leuven, Belgium.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't