Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1990-6-20
|
| pubmed:databankReference | |
| pubmed:abstractText |
Our laboratory recently isolated a cDNA for cytochrome P-450g (IIC13), a male-specific, highly polymorphic P-450 isozyme, from livers of the high phenotype (+g) of Sprague-Dawley rats [McClellan-Green et al. (1989) Biochemistry 28, 5832-5839]. Hybridization studies using a specific oligonucleotide probe for P-450 (+g) indicated that equivalent amounts of P-450g mRNA were present in livers of both the high and low phenotypes (+g and -g) of male Sprague-Dawley, Fischer (inbred -g), or ACI (inbred +g) rats. In the present study, we isolated one full-length and one nearly full-length cDNA clone coding for the unexpressed form of cytochrome P-450g from a cDNA library constructed from mRNA from a (-g) male Sprague-Dawley rat. The longest cDNA had an open reading frame of 1473 nucleotides which coded for a 490 amino acid polypeptide of Mr 55,839. Although the 5'-noncoding leader sequence and the 3'-noncoding region were unchanged, the coding sequence of the (-g) phenotype differed from that of the cDNA isolated from the (+g) phenotype by nine bases changes. These base changes would result in seven amino acid differences between the protein sequences for the two phenotypes. Two specific oligonucleotide probes for (+) P-450g and (-) P-450g containing three base differences between the (+g) and (-g) sequences hybridized differentially to mRNA from the (+g) and (-g) phenotypes.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0006-2960
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
23
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
713-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2337591-Amino Acid Sequence,
pubmed-meshheading:2337591-Animals,
pubmed-meshheading:2337591-Base Sequence,
pubmed-meshheading:2337591-Blotting, Northern,
pubmed-meshheading:2337591-Cloning, Molecular,
pubmed-meshheading:2337591-Cytochrome P-450 Enzyme System,
pubmed-meshheading:2337591-DNA,
pubmed-meshheading:2337591-Female,
pubmed-meshheading:2337591-Isoenzymes,
pubmed-meshheading:2337591-Liver,
pubmed-meshheading:2337591-Male,
pubmed-meshheading:2337591-Molecular Sequence Data,
pubmed-meshheading:2337591-Nucleic Acid Hybridization,
pubmed-meshheading:2337591-Oligonucleotides,
pubmed-meshheading:2337591-Phenotype,
pubmed-meshheading:2337591-Polymorphism, Genetic,
pubmed-meshheading:2337591-RNA, Messenger,
pubmed-meshheading:2337591-Rats,
pubmed-meshheading:2337591-Rats, Inbred Strains
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Characterization of a cDNA for the unexpressed form of cytochrome P-450g from the (-g) rat and differentiation of its mRNA from that of the (+g) phenotype using specific oligoprobes.
|
| pubmed:affiliation |
National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|