| pubmed-article:2320376 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0015127 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0040688 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0040845 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C1314792 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0751364 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C1519697 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:2320376 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:2320376 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:2320376 | pubmed:dateCreated | 1990-5-10 | lld:pubmed |
| pubmed-article:2320376 | pubmed:abstractText | The human teratocarcinoma NTERA-2 cl. D1 (NT2/D1) cell is a cloned multipotential embryonal cancer cell line that differentiates into a neuronal phenotype and other cellular lineages with retinoic acid (RA) treatment. Here we report that mRNA for the transforming growth factor-alpha is expressed in these RA-untreated cells and that RA-treatment results in a reduction of mRNA expression within 24 hr of treatment. In total cellular RNA, TGF-alpha mRNA is not detectable by Northern analysis at 6 days when there is increased expression of the human homeotic genes Hu-1 (Hox 2.1) and Hu-2 (Hox 2.2), known markers of RA response in NT2/D1 cells. RA treatment also causes a marked reduction in cloning efficiency and tumorigenicity of these cells. The addition of TGF-alpha or EGF (epidermal growth factor) protein to RA-untreated NT2/D1 cells augments soft agar cloning under limited fetal calf serum conditions. Blocking monoclonal antibodies directed against the EGF receptor (EGFr) can prevent this augmentation. We conclude that TGF-alpha expression inversely correlates with the state of RA-induced differentiation of this human teratocarcinoma cell and that TGF-alpha and EGF proteins are stimulatory growth factors in NT2/D1 cells under these culture conditions. | lld:pubmed |
| pubmed-article:2320376 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2320376 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2320376 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2320376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2320376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2320376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2320376 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2320376 | pubmed:issn | 0890-6467 | lld:pubmed |
| pubmed-article:2320376 | pubmed:author | pubmed-author:MasuiHH | lld:pubmed |
| pubmed-article:2320376 | pubmed:author | pubmed-author:MAHH | lld:pubmed |
| pubmed-article:2320376 | pubmed:author | pubmed-author:MillerW HWHJr | lld:pubmed |
| pubmed-article:2320376 | pubmed:author | pubmed-author:MorCC | lld:pubmed |
| pubmed-article:2320376 | pubmed:author | pubmed-author:DmitrovskyEE | lld:pubmed |
| pubmed-article:2320376 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2320376 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:2320376 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2320376 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2320376 | pubmed:pagination | 233-9 | lld:pubmed |
| pubmed-article:2320376 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:meshHeading | pubmed-meshheading:2320376-... | lld:pubmed |
| pubmed-article:2320376 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2320376 | pubmed:articleTitle | Retinoic acid causes a decline in TGF-alpha expression, cloning efficiency, and tumorigenicity in a human embryonal cancer cell line. | lld:pubmed |
| pubmed-article:2320376 | pubmed:affiliation | Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York 10021. | lld:pubmed |
| pubmed-article:2320376 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2320376 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2320376 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2320376 | lld:pubmed |
