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rdf:type |
|
|
lifeskim:mentions |
|
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pubmed:issue |
1
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pubmed:dateCreated |
1990-4-19
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pubmed:abstractText |
A method for the in vitro immunization of human tonsilar lymphocytes is described. This method allows for the sensitization of B-cells against various antigens under serum-free medium conditions. The medium is supplemented with defined concentrations of antigen, rIL-2 and muramyl dipeptide to boost the immune responses. This method may prove useful for the generation of antigen reactive B-cells for use in human monoclonal antibody production and the study of the effects of other lymphokines on the in vitro immune response.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Feb
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|
pubmed:issn |
0272-457X
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
9
|
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pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
81-9
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|
pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2312133-Acetylmuramyl-Alanyl-Isoglutamine,
pubmed-meshheading:2312133-Antibodies, Monoclonal,
pubmed-meshheading:2312133-Antigens,
pubmed-meshheading:2312133-Culture Media,
pubmed-meshheading:2312133-Humans,
pubmed-meshheading:2312133-Immunization,
pubmed-meshheading:2312133-Interleukin-2,
pubmed-meshheading:2312133-Lymphocytes,
pubmed-meshheading:2312133-Palatine Tonsil,
pubmed-meshheading:2312133-T-Lymphocytes,
pubmed-meshheading:2312133-Time Factors
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|
pubmed:year |
1990
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|
pubmed:articleTitle |
Parameters involved in the in vitro immunization of tonsilar lymphocytes: effects of rIL-2 and muramyl dipeptide.
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pubmed:affiliation |
Bio Research Ireland, Dublin City University.
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pubmed:publicationType |
Journal Article,
In Vitro
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