Linked Life Data

2310031

Source:http://linkedlifedata.com/resource/pubmed/id/2310031

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-4-5
pubmed:abstractText
Single-channel recording techniques have been used to examine interactions between anticholinesterases and ion channels activated by acetylcholine. Single-channel currents activated by 200 nM acetylcholine were recorded from cell-attached patches of BC3H1 mouse tumor cells grown in culture. Channels were recorded in the absence and presence of edrophonium (1-20 microM), neostigmine (2-20 microM), or pyridostigmine (10-200 microM). All three drugs shortened channel open time but did not alter single-channel current amplitude. Effects on channel open time were not secondary to inhibition of cholinesterase but appeared to involve direct interactions between anticholinesterase drugs and acetylcholine-activated channels. Drug concentrations calculated to reduce the time constant of open time distributions by 50% were 3.8 microM edrophonium, 4.6 microM neostigmine, or 97 microM pyridostigmine. Channel open time was decreased by edrophonium at concentrations comparable to those occurring during reversal of neuromuscular block, but it was reduced by neostigmine and pyridostigmine only at levels higher than those encountered clinically. Differences in interactions between anticholinesterases and acetylcholine-activated channels at the end plate may possibly account for some of the clinical differences between these drugs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0003-3022
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
496-503
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Comparison of anticholinesterases and their effects on acetylcholine-activated ion channels.
pubmed:affiliation
Veterans Administration Medical Center, Iowa City, Iowa 52246.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't