Linked Life Data

2288734

Source:http://linkedlifedata.com/resource/pubmed/id/2288734

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1991-4-1
pubmed:abstractText
A sensitive method is discribed for the diagnosis of alcoholism. The method is based on the detection of a transferrin variant (carbohydrate deficient transferrin = CDT) in plasma of alcoholics. The determination of CDT, the presence of which is characteristic for chronic alcoholism, is performed in three steps: The plasma proteins are separated by polyacrylamide gel electrophoresis and then transferred electrophoretically onto a nitrocellulose sheet; finally, the transferrin types on the nitrocellulose sheet are specifically detected by an antibody reaction. With the exception of certain cases (genetic variants, rare diseases) CDT is found only during chronic alcohol consumption. In comparison to other markers for chronic alcoholism an advantage of CDT is its higher specificity. A further advantage of the method is that CDT can be identified with high sensitivity by the use of a relatively small amount of technical equipment.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-5250
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
427-33
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
[Diagnosis of alcoholism based on detection of a transferrin variant by polyacrylamide gel electrophoresis and immunoblotting].
pubmed:affiliation
Anatomischen Anstalt, Lehrstuhl II, Ludwig-Maximilians-Universität München.
pubmed:publicationType
Journal Article, English Abstract