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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1991-3-15
|
| pubmed:abstractText |
We have characterized a chromosomal translocation in a cell line (SU-DUL5) established from a patient with lymphoblastic lymphoma in which the c-myc gene on chromosome 8 was juxtaposed to a t(14;18). Cytogenetic analysis of this cell line showed 14q+, 18q-, and 8p+q+ marker chromosomes in the absence of t(14;18). Genomic Southern blot analysis showed juxtaposition of the immunoglobulin heavy chain joining region (JH) with chromosome 18 near the minor breakpoint cluster region (mcr) of the bcl-2 gene. There was also a rearranged c-myc gene detectable with a 5' c-myc probe. Molecular cloning studies showed that the c-myc gene was joined to chromosome 18 DNA. Nucleotide sequence analysis of cloned breakpoint DNA revealed that the crossover between chromosomes 8 and 18 occurred at the 3' end of the bcl-2 gene resulting in replacement of the bcl-2 gene on the 14q+ chromosome with the c-myc gene. As a result of this translocation the SU-DUL5 cell line contains no detectable bcl-2 mRNA or protein but has abundant levels of c-myc mRNA. Our data suggest that bcl-2 inactivation occurred simultaneously with c-myc translocation in a B cell lymphoblastic lymphoma.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:geneSymbol |
bcl-2,
c-myc
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1815-9
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2284100-Adult,
pubmed-meshheading:2284100-Base Sequence,
pubmed-meshheading:2284100-Blotting, Northern,
pubmed-meshheading:2284100-Cell Line,
pubmed-meshheading:2284100-Chromosomes, Human, Pair 14,
pubmed-meshheading:2284100-Chromosomes, Human, Pair 18,
pubmed-meshheading:2284100-Chromosomes, Human, Pair 8,
pubmed-meshheading:2284100-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:2284100-Genes, myc,
pubmed-meshheading:2284100-Humans,
pubmed-meshheading:2284100-Karyotyping,
pubmed-meshheading:2284100-Male,
pubmed-meshheading:2284100-Molecular Sequence Data,
pubmed-meshheading:2284100-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:2284100-Proto-Oncogene Proteins,
pubmed-meshheading:2284100-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:2284100-Proto-Oncogenes,
pubmed-meshheading:2284100-RNA, Messenger,
pubmed-meshheading:2284100-Translocation, Genetic
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Concurrent activation of c-myc and inactivation of bcl-2 by chromosomal translocation in a lymphoblastic lymphoma cell line.
|
| pubmed:affiliation |
Division of Hematology, Stanford University School of Medicine, California 94305.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|