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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1980-1-28
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pubmed:abstractText |
Total RNA or poly(A)(+) RNA of rat liver was translated in a rabbit reticulocyte or wheat germ protein-synthesizing system and the carbamyl phosphate synthetase I [carbamoyl-phosphate synthetase (ammonia); carbon dioxide: ammonia ligase (ADP-forming, carbamate-phosphorylating), EC 6.3.4.16] synthesized was isolated by indirect immunoprecipitation by using antibody purified on enzyme-bound Sepharose and Staphylococcus aureus cells. The in vitro product moved on sodium dodecyl sulfate/polyacrylamide gels as a polypeptide that was about 5000 daltons larger than the subunit of the mature enzyme (160,000 daltons). The same polypeptide was also obtained by direct immunoprecipitation or by a double-antibody precipitation method. The mature enzyme competed effectively with the in vitro product for interaction with anti-carbamyl phosphate synthetase I antibody. Digestion of the in vitro product by S. aureus protease gave a pattern of peptide fragments similar to that of the mature enzyme. A mitochondrial membrane preparation from rat liver converted the in vitro product into a polypeptide that comigrated with the mature subunit on sodium dodecyl sulfate gel electrophoresis. Similar proteolytic activity was not detected in either a cytosol or a microsomal fraction of rat liver. These results indicate that the enzyme is synthesized as a larger precursor which is converted to the mature form of enzyme by posttranslational processing.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-1102604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-1148171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-14283188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-154672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-175068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-182079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-188032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-206435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-218964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-226957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-265554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-320200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4310091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4525168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4858491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4908238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4959756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-4971842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-5100767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-5660075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-5751125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-790996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/228276-942098
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5071-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:228276-Animals,
pubmed-meshheading:228276-Carbamoyl-Phosphate Synthase (Ammonia),
pubmed-meshheading:228276-Intracellular Membranes,
pubmed-meshheading:228276-Mitochondria, Liver,
pubmed-meshheading:228276-Phosphotransferases,
pubmed-meshheading:228276-Plants,
pubmed-meshheading:228276-Poly A,
pubmed-meshheading:228276-Protein Biosynthesis,
pubmed-meshheading:228276-RNA,
pubmed-meshheading:228276-Rabbits,
pubmed-meshheading:228276-Rats,
pubmed-meshheading:228276-Reticulocytes,
pubmed-meshheading:228276-Triticum
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pubmed:year |
1979
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pubmed:articleTitle |
Cell-free synthesis and processing of a putative precursor for mitochondrial carbamyl phosphate synthetase I of rat liver.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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