Linked Life Data

2275531

Source:http://linkedlifedata.com/resource/pubmed/id/2275531

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1991-2-28
pubmed:databankReference
pubmed:abstractText
Blood samples to be tested for the presence of parasite DNA by using specific DNA probes are routinely stored in our laboratory as high-salt lysates (HSL). To safeguard against the risk of accidental infection with etiological agents such as the human immunodeficiency virus type 1 (HIV-1) while manipulating large numbers of blood samples in preparation for DNA probing, we determined the residual infectivity of HIV-1 after exposure to HSL components. Both high-titer virus stocks or provirus-carrying cells, suspended either in tissue culture medium or freshly drawn blood, were completely inactivated upon contact with the HSL components. This was verified by the absence of any detectable HIV-1-specific antigen in the supernatants of long-term cultures and the absence of virus-specific DNA fragments after amplification by polymerase chain reaction with DNA from such cultures as target DNA. These results support the conclusion that the virus is in fact completely inactivated by contact with the HSL components, rendering blood specimens stored as HSL noninfectious in regard to HIV-1.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-2181308, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-2644856, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-2677719, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-3014036, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-3041857, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-3094962, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-3285726, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-3336784, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-3472723, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-3492810, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-5669470, http://linkedlifedata.com/resource/pubmed/commentcorrection/2275531-6606682
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2755-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Inactivation of human immunodeficiency virus type 1 in blood samples stored as high-salt lysates.
pubmed:affiliation
Department of Molecular Biology, Biomedical Research Institute, Rockville, Maryland 20852.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, Non-P.H.S.