| pubmed-article:2250044 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C0242275 | lld:lifeskim |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C1518174 | lld:lifeskim |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C0040688 | lld:lifeskim |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C1948023 | lld:lifeskim |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C1519667 | lld:lifeskim |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C0205228 | lld:lifeskim |
| pubmed-article:2250044 | lifeskim:mentions | umls-concept:C1527234 | lld:lifeskim |
| pubmed-article:2250044 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2250044 | pubmed:dateCreated | 1991-1-15 | lld:pubmed |
| pubmed-article:2250044 | pubmed:abstractText | Carcinoma cells frequently coexpress transforming growth factor (TGF)-alpha and its receptor, the epidermal growth factor (EGF) receptor, implicating an autocrine function of carcinoma-derived TGF-alpha. Using a monoclonal antibody (425) to the EGF-receptor, we investigated the role of exogenous and tumor cell-derived EGF/TGF-alpha mitogenic activities in proliferation of cell lines derived from solid tumors. Monoclonal antibody 425 was chosen for these studies because it inhibits binding of EGF/TGF-alpha to the EGF-receptor and effectively blocks activation of the EGF-receptor by EGF/TGF-alpha. Seven malignant cell lines originating from carcinomas of colon, pancreas, breast, squamous epithelia, and bladder expressed surface EGF-receptor and secreted EGF/TGF-alpha-like mitogenic activities into their tissue culture media. All cell lines were maintained in a defined medium free of exogenous EGF/TGF-alpha. EGF and TGF-alpha added to the culture medium stimulated proliferation of five cell lines to comparable levels. EGF/TGF-alpha-dependent proliferation was significantly reduced by addition of MAb 425 to culture media. In addition, monoclonal antibody 425 reduced proliferation of the five EGF/TGF-alpha responsive cell lines in the absence of exogenous EGF/TGF-alpha. Antiproliferative effects induced by monoclonal antibody 425 were reversible and could be overcome by addition of EGF to culture media. Our results indicate that tumor-derived EGF-receptor-reactive mitogens can promote proliferation of carcinoma cells in an autocrine fashion. | lld:pubmed |
| pubmed-article:2250044 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2250044 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2250044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2250044 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2250044 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2250044 | pubmed:issn | 0730-2312 | lld:pubmed |
| pubmed-article:2250044 | pubmed:author | pubmed-author:WilliamsNN | lld:pubmed |
| pubmed-article:2250044 | pubmed:author | pubmed-author:HerlynMM | lld:pubmed |
| pubmed-article:2250044 | pubmed:author | pubmed-author:RodeckUU | lld:pubmed |
| pubmed-article:2250044 | pubmed:author | pubmed-author:MurthaMM | lld:pubmed |
| pubmed-article:2250044 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2250044 | pubmed:volume | 44 | lld:pubmed |
| pubmed-article:2250044 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2250044 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2250044 | pubmed:pagination | 69-79 | lld:pubmed |
| pubmed-article:2250044 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:meshHeading | pubmed-meshheading:2250044-... | lld:pubmed |
| pubmed-article:2250044 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2250044 | pubmed:articleTitle | Monoclonal antibody 425 inhibits growth stimulation of carcinoma cells by exogenous EGF and tumor-derived EGF/TGF-alpha. | lld:pubmed |
| pubmed-article:2250044 | pubmed:affiliation | Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104. | lld:pubmed |
| pubmed-article:2250044 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2250044 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2250044 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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