|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
32
|
|
pubmed:dateCreated |
1991-1-8
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The complete structure of the mouse 68-kDa neurofilament (NF-L) gene was elucidated. We cloned cDNAs corresponding to 3.5- and 2.3-kb NF-L mRNA, including their polyadenylation sites. Sequence analysis revealed that these NF-L mRNAs arose from the alternative use of two polyadenylation sites in exon 4. Promoter analysis using NF-L promoter-beta-galactosidase fusion plasmids determined regions responsible for its basic promoter activity, which were located between -328 and -36 base pairs from the transcription initiation site. These promoter fusion plasmids induced a significant level of beta-galactosidase in NF-nonproducing C6 cells as well as in NF-producing PC12h cells. The in vitro transcription assay using HeLa cell extract also showed that this promoter exhibited strong transcriptional activity. Little difference in NF-L mRNA stability was observed between the two cells. However, nuclear run-off assay revealed that the NF-L gene was not transcribed in NF-nonproducing C6 cells. These data suggest that the strong promoter activity of the NF-L gene is repressed in vivo at the transcription initiation level in a tissue-specific manner.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
265
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
19786-91
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:2246261-Animals,
pubmed-meshheading:2246261-Base Sequence,
pubmed-meshheading:2246261-Binding Sites,
pubmed-meshheading:2246261-Cloning, Molecular,
pubmed-meshheading:2246261-Deoxyribonuclease EcoRI,
pubmed-meshheading:2246261-Exons,
pubmed-meshheading:2246261-Gene Expression Regulation,
pubmed-meshheading:2246261-HeLa Cells,
pubmed-meshheading:2246261-Humans,
pubmed-meshheading:2246261-Intermediate Filament Proteins,
pubmed-meshheading:2246261-Mice,
pubmed-meshheading:2246261-Molecular Sequence Data,
pubmed-meshheading:2246261-Neurofilament Proteins,
pubmed-meshheading:2246261-Nucleic Acid Hybridization,
pubmed-meshheading:2246261-Poly A,
pubmed-meshheading:2246261-Promoter Regions, Genetic,
pubmed-meshheading:2246261-RNA, Messenger,
pubmed-meshheading:2246261-Restriction Mapping,
pubmed-meshheading:2246261-Transcription, Genetic,
pubmed-meshheading:2246261-Transfection
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Structure of the 68-kDa neurofilament gene and regulation of its expression.
|
|
pubmed:affiliation |
Division of Regulation of Macromolecular Functions, Osaka University, Japan.
|
|
pubmed:publicationType |
Journal Article
|
