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rdf:type |
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lifeskim:mentions |
umls-concept:C0033684,
umls-concept:C0079429,
umls-concept:C0086418,
umls-concept:C0229671,
umls-concept:C0332162,
umls-concept:C0443146,
umls-concept:C0949374,
umls-concept:C1155631,
umls-concept:C1334043,
umls-concept:C1826786,
umls-concept:C2700640
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pubmed:issue |
21
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pubmed:dateCreated |
1990-12-7
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pubmed:abstractText |
Several autoimmune sera from patients with Raynaud phenomenon decorated mammalian kinetochores and bound to a 47-kDa protein on immunoblots of nuclear lysates. Antibody affinity-purified from immunoblots of the 47-kDa band recognized kinetochores, but due to crossreaction with an 18-kDa protein, localization remains elusive. We used one of these sera to purify the antigen from HeLa cells synchronized in mitosis as a noncovalent complex with a 25-kDa protein. The antigen was released from DNA by intercalation with 25 mM chloroquine. Ion-exchange chromatography yielded the pure complex with an apparent molecular size of 68 kDa, which was separated into its components by gel filtration in 6 M guanidinium chloride. Upon two-dimensional gel electrophoresis the 47-kDa protein gave two main spots of pI 6.6 and 6.7, respectively. Posttranslational modification is indicated by additional antigenic spots, by lack of a free alpha-amino group, and by chromatographic behavior of peptides on reversed-phase chromatography. The amino acid sequence for 205 residues of the 47-kDa protein has been established. This sequence is highly homologous with the translated reading frame of RCC1, a gene reportedly involved in regulating onset of mammalian chromosome condensation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-2435739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-2507317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-2579778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-2651051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-2677018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-2808515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3305522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3315496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3339086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3340202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3531335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3550420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3608716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3678831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-3793763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-4092697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-4571311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-5912064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-6383665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-6605200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-6966403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-6968511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2236072-7298727
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:geneSymbol |
RCC1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8617-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2236072-Amino Acid Sequence,
pubmed-meshheading:2236072-Autoantibodies,
pubmed-meshheading:2236072-Autoantigens,
pubmed-meshheading:2236072-Autoimmune Diseases,
pubmed-meshheading:2236072-Chromosomes, Human,
pubmed-meshheading:2236072-Female,
pubmed-meshheading:2236072-Fluorescent Antibody Technique,
pubmed-meshheading:2236072-Genes,
pubmed-meshheading:2236072-HeLa Cells,
pubmed-meshheading:2236072-Humans,
pubmed-meshheading:2236072-Middle Aged,
pubmed-meshheading:2236072-Molecular Sequence Data,
pubmed-meshheading:2236072-Molecular Weight,
pubmed-meshheading:2236072-Nuclear Proteins,
pubmed-meshheading:2236072-Raynaud Disease,
pubmed-meshheading:2236072-Sequence Homology, Nucleic Acid
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pubmed:year |
1990
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pubmed:articleTitle |
A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation.
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pubmed:affiliation |
Institute of Cell and Tumor Biology, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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