Linked Life Data

222581

Source:http://linkedlifedata.com/resource/pubmed/id/222581

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1979-9-25
pubmed:abstractText
We present here a differentiation by kinetic methods of the tandem processes of transport and metabolic during uptake of cytosine-beta-D-arabinoside by intact rat fibroblasts. Transport across the cell membrane occurs by a carrier-mediated mechanism displaying a Km of approximately 500 microM and a V of approximately pmol x min-1 x (10(6) cells)-1. The subsequent metabolic trapping (phosphorylation) has a Km of approximately 15 microM and V of approximately 0.25 pmol x min-1 x (10(6) cells)-1. In this system, transport is rate-limiting for the first phase of the uptake process whereas phosphorylation becomes rate-limiting when internal concentration of radioactive labeled substrate exceeds that in the extracellular medium. The duration of the first phase depends on the substrate concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
333-9
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
A kinetic analysis of the uptake of cytosine-beta-D-arabinoside by rat-B77 cells. Differentiation between transport and phosphorylation.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.