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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0021756,
umls-concept:C0024264,
umls-concept:C0027651,
umls-concept:C0041904,
umls-concept:C0123771,
umls-concept:C0162493,
umls-concept:C0185117,
umls-concept:C0332448,
umls-concept:C0591833,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1990-10-11
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pubmed:abstractText |
Since interleukin-2 (IL-2) and IL-4 act in concert to support the development of cytotoxic T lymphocytes (CTL) and the generation of antigen-specific tumour infiltrating lymphocytes (TIL), we investigated the interaction of these cytokines with an established TIL line. TIL proliferated in an additive fashion in response to suboptimal concentrations of IL-2 and various concentrations of IL-4. TIL possessed high-affinity IL-4 receptors whether cultured in recombinant IL-2 (rIL-2) or rIL-4, but cells cultured in rIL-2 had higher numbers of IL-4 receptors than cells cultured in rIL-4. When TIL were cultured in increasing concentrations of rIL-2, a dose-dependent enhancement in IL-4 receptor number was observed. The maximum induction of IL-4 receptor expression was achieved by 4 hr of incubation with rIL-2 and was completely blocked by cycloheximide. Other cytokines, such as rIL-1, recombinant tumour necrosis factor (rTNF), recombinant interferon-alpha (rIFN-alpha) and rIFN-gamma, had no effect on IL-4 receptor number. rIL-2 also up-regulated IL-4 receptors on CTLL-2, a murine CTL line. These data indicate that high-affinity IL-4 receptors exist on murine TIL and they can be up-regulated by IL-2. Our observation that IL-2 up-regulates IL-4 receptor may help explain the additive effects of these lymphokines on the proliferation of TIL and other cell lines. It may also help explain their co-operative effects on the generation of antigen-specific TIL and the differentiation of CTL.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-2461987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-2521354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-2529125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-2961813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-2970518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3100623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3158703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3262710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3263648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3264324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3264384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3330541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3489291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3493432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3494245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3500355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-3875569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-6976792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-6977612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2203676-7284236
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
492-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2203676-Animals,
pubmed-meshheading:2203676-Biological Factors,
pubmed-meshheading:2203676-Cell Division,
pubmed-meshheading:2203676-Cytokines,
pubmed-meshheading:2203676-Interleukin-2,
pubmed-meshheading:2203676-Interleukin-4,
pubmed-meshheading:2203676-Mice,
pubmed-meshheading:2203676-Mice, Inbred C57BL,
pubmed-meshheading:2203676-Neoplasms, Experimental,
pubmed-meshheading:2203676-Receptors, Interleukin-4,
pubmed-meshheading:2203676-Receptors, Mitogen,
pubmed-meshheading:2203676-Recombinant Proteins,
pubmed-meshheading:2203676-T-Lymphocytes,
pubmed-meshheading:2203676-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:2203676-Tumor Cells, Cultured
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pubmed:year |
1990
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pubmed:articleTitle |
Expression of high-affinity IL-4 receptors on murine tumour infiltrating lymphocytes and their up-regulation by IL-2.
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pubmed:affiliation |
Division of Cytokine Biology, CBER/FDA, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article
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