Linked Life Data

2200540

Source:http://linkedlifedata.com/resource/pubmed/id/2200540

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-9-18
pubmed:abstractText
The ontogeny of a 57-Kd cationic antimicrobial protein (CAP57) that has substantial similarities to bactericidal permeability increasing protein (BPI) has been determined immunocytochemically. CAP57 was detected in the granules of mature peripheral blood neutrophils. However, it was absent from other cells of the peripheral blood: eosinophils, red blood cells (RBCs), and mononuclear cells. In human bone marrow, CAP57 was confined to the neutrophilic series. The earliest stage of development of the myeloid cells at which CAP57 was demonstrated was the promyelocyte. Double immunofluorescent labeling showed that CAP57 was detected in cells positive for myeloperoxidase. The absence of lactoferrin in certain cells (promyelocytes) containing CAP57 indicated that CAP57 was synthesized and packaged in a population of granules prior to the development of granules that contain lactoferrin. CAP57 could not be demonstrated in HL60 cells either by enzyme-linked immunosorbent assay (ELISA) or by immunocytochemistry. However, the presence of another granule-associated cationic antimicrobial protein of molecular weight 37 Kd (CAP37) was readily detected in undifferentiated HL60 cells. Amino acid sequence analysis showed that CAP57 and BPI were identical. Further indication of the identity between CAP57 and BPI was that monoclonal anti-CAP57 antibodies cross reacted with BPI. Sucrose density-gradient centrifugations showed CAP57 was confined to a granule population that exhibited a buoyant density intermediate of the previously described light and heavy azurophil granules. Further resolution of the individual azurophil granule populations by Percoll density-gradient centrifugation revealed that CAP57 was most concentrated in the density range of 1.093 to 1.100 g/cc. These results strongly suggest the unique finding that CAP57 may be associated with a heretofore unreported granule type.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
825-34
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2200540-Amino Acid Sequence, pubmed-meshheading:2200540-Amino Acids, pubmed-meshheading:2200540-Antibodies, Monoclonal, pubmed-meshheading:2200540-Antimicrobial Cationic Peptides, pubmed-meshheading:2200540-Blood Proteins, pubmed-meshheading:2200540-Bone Marrow, pubmed-meshheading:2200540-Bone Marrow Cells, pubmed-meshheading:2200540-Cell Line, pubmed-meshheading:2200540-Centrifugation, Density Gradient, pubmed-meshheading:2200540-Cross Reactions, pubmed-meshheading:2200540-Cytoplasmic Granules, pubmed-meshheading:2200540-Fluorescent Antibody Technique, pubmed-meshheading:2200540-Humans, pubmed-meshheading:2200540-Immunohistochemistry, pubmed-meshheading:2200540-Leukemia, pubmed-meshheading:2200540-Membrane Proteins, pubmed-meshheading:2200540-Molecular Sequence Data, pubmed-meshheading:2200540-Neutrophils
pubmed:year
1990
pubmed:articleTitle
The ontogeny of a 57-Kd cationic antimicrobial protein of human polymorphonuclear leukocytes: localization to a novel granule population.
pubmed:affiliation
Department of Microbiology, Emory University School of Medicine, Atlanta, GA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.