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rdf:type |
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lifeskim:mentions |
umls-concept:C0020291,
umls-concept:C0031621,
umls-concept:C0036098,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0322495,
umls-concept:C0439849,
umls-concept:C0458083,
umls-concept:C0596233,
umls-concept:C1704353,
umls-concept:C1879547
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pubmed:issue |
1
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pubmed:dateCreated |
1979-6-11
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pubmed:abstractText |
The addition of 5-hydroxytryptamine to the isolated blowfly salivary gland stimulates fluid secretion, transepithelial calcium transport and the breakdown of 32P- or 3H-labelled phosphatidylinositol The breakdown of [32P]phosphatidylcholine and [32P]-phosphatidylethanolamine was not stimulated by 5-hydroxytryptamine. In salivary glands incubated with myo-[2-3H]inositol for 1--3 h, more than 95% of the label retained by the tissue was in the form of phosphatidylinositol. The addition of 5-hydroxytryptamine resulted in an increase in the accumulation of label in intracellular inositol 1:2-cyclic phosphate, inositol 1-phosphate and free inositol along with an increase in the release of [3H]inositol to the medium and saliva. The release of [3H]inositol to the medium served as a sensitive indicator of phosphatidylinositol breakdown. The release of [3H]inositol was not increased by cyclic AMP or the bivalent-cation ionophore A23187 under conditions in which salivary secretion was accelerated. The stimulation of fluid secretion by low concentrations of 5-hydroxytryptamine was potentiated by 3-isobutyl-1-methylxanthine, which had no effect on inositol release. The stimulation of fluid secretion by 5-hydroxytryptamine was greatly reduced in calcium-free buffer, but the breakdown of phosphatidylinositol continued at the same rate in the absence of calcium. These results support the hypothesis that breakdown of phosphatidylinositol by 5-hydroxytryptamine is involved in the gating of calcium.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-1107025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-13192107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-13641241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-13715209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-13814585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-14097353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-163953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-164246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-171926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-173284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-175090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-178681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-330283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-335830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-4323007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-4330852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-4335064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-435285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-4872349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-5426559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-5483450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-5721468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-84769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-856711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-920473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219851-992019
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
45-58
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:219851-Animals,
pubmed-meshheading:219851-Biological Transport,
pubmed-meshheading:219851-Calcium,
pubmed-meshheading:219851-Cyclic AMP,
pubmed-meshheading:219851-Diptera,
pubmed-meshheading:219851-Female,
pubmed-meshheading:219851-Hydrolysis,
pubmed-meshheading:219851-Inositol,
pubmed-meshheading:219851-Inositol Phosphates,
pubmed-meshheading:219851-Phosphatidylinositols,
pubmed-meshheading:219851-Phospholipids,
pubmed-meshheading:219851-Salivary Glands,
pubmed-meshheading:219851-Secretory Rate,
pubmed-meshheading:219851-Serotonin
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pubmed:year |
1979
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pubmed:articleTitle |
Relationship between hormonal activation of phosphatidylinositol hydrolysis, fluid secretion and calcium flux in the blowfly salivary gland.
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pubmed:publicationType |
Journal Article,
In Vitro
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