Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-8-14
pubmed:abstractText
Non-surgically collected day 7 bovine embryos were microsurgically cleaved and reinserted into surrogate zonae pellucidae. The demi-embryos were fixed immediately after splitting and at various intervals of in vitro incubation at 35 degrees C in modified Dulbecco's medium. At the light-microscopical level, the demi-embryos were found to have restored their prebisection morphology within 30 min after splitting. The electron microscopy confirmed these findings, by the presence of mitosis, cell polarization, neoformation and reaggregation of mitochondria, increased pinocytosis and cell-to-cell contact between trophoblastic and/or inner cell mass cells. The present observations therefore suggest that day 7 bovine demi-embryos intended for transfer should be cultured for 30 min before being morphologically evaluated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0001-5180
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Morphology of day 7 bovine demi-embryos during in vitro reorganization.
pubmed:affiliation
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't