Linked Life Data

21899439

Source:http://linkedlifedata.com/resource/pubmed/id/21899439

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2011-9-8
pubmed:abstractText
Analysis of pathological and molecular data of 'Candidatus Phytoplasma mali' accessions from 27 apple trees differing considerably in symptomatology was used to molecularly characterize and classify strains of the infecting apple proliferation phytoplasma. Single-strand conformation polymorphism and sequence analysis of a variable fragment of ATP00464-type hflB gene revealed that these sources consisted of single-strain and multiple-strain accessions that occurred in similar numbers. The latter group was composed of two to five distinct strains. Analysis of cloned sequences of mild and severe single-strain accessions resulted in two groups of reads that clustered, according to their virulence, distantly in the phylogram. Based on this data, the clustering patterns of multiple-strain accession sequences indicated that nearly all of them were composed of mild and severe strains. The distinct clustering of sequences representing mild and severe strains was associated with a range of molecular markers at the nucleotide and amino acid level. Data indicate that the virulence of multiple-strain accessions is determined by the ratio of the occurring mild and severe strains in that mild accessions were characterized by the predominance of sequences representing mild strains and vice versa. There is evidence that shifts in the population and other events may occur that drastically alter virulence of multiple-strain accessions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0894-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1258-66
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
HflB gene-based phytopathogenic classification of 'Candidatus phytoplasma mali' strains and evidence that strain composition determines virulence in multiply infected apple trees.
pubmed:affiliation
Julius Kuehn Institute, Federal Research Center for Cultivated Palnts, Institute for Plant Protection in Fruuit Crops, Germany. erich.seemueller@jki.bund.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't