Linked Life Data

2184252

Source:http://linkedlifedata.com/resource/pubmed/id/2184252

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1990-5-30
pubmed:abstractText
We have serially investigated the role of nutrients and growth factors for their ability to support the clonal growth of human bladder tumor cell lines. Five established human bladder tumor cell lines were selected for study of their requirements for clonogenic growth in semisolid cultures. We identified an "optimal medium" to be Alpha MEM medium, 15% horse serum, five micrograms/ml. of transferrin, five micrograms/ml. of insulin and 30 ng./ml. of epidermal growth factor. The "optimal assay" enhanced the growth of human bladder tumor cell lines 2.5 times compared to the standard clonogenic assay. The resulting medium is less complicated to maintain, less expensive and supports the growth of human bladder tumor cell lines better than the standard clonogenic assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-5347
pubmed:author
pubmed:issnType
Print
pubmed:volume
143
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1049-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Clonal growth requirements of human bladder tumor cell lines.
pubmed:affiliation
Veterans Administration Research Service, V. A. Medical Center, Memphis, Tennessee.
pubmed:publicationType
Journal Article, Comparative Study