Linked Life Data

21821894

Source:http://linkedlifedata.com/resource/pubmed/id/21821894

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 8
pubmed:dateCreated
2011-8-8
pubmed:abstractText
Glucokinase catalyzes the phosphorylation of glucose using ATP to yield glucose 6-phosphate. SgGlkA is a bacterial group III glucokinase from Streptomyces griseus that seems to play a regulatory role in carbon catabolite repression in this organism. SgGlkA was expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method at 293?K. A crystal of SgGlkA in complex with glucose was obtained using a reservoir solution consisting of 0.9?M sodium/potassium tartrate, 0.2?M NaCl and 0.1?M imidazole pH 8.1 and diffracted X-rays to 1.84?Å resolution. The crystal of SgGlkA in complex with glucose belonged to space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 109.19, c = 141.18?Å. The crystal contained one molecule in the asymmetric unit.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1744-3091
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
914-6
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose.
pubmed:affiliation
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't