Linked Life Data

21775598

Source:http://linkedlifedata.com/resource/pubmed/id/21775598

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
2011-7-21
pubmed:abstractText
Orexin/hypocretin neurons have a crucial role in the regulation of sleep and wakefulness. To help determine how these neurons promote wakefulness, we generated transgenic mice in which orexin neurons expressed halorhodopsin (orexin/Halo mice), an orange light-activated neuronal silencer. Slice patch-clamp recordings of orexin neurons that expressed halorhodopsin demonstrated that orange light photic illumination immediately hyperpolarized membrane potential and inhibited orexin neuron discharge in proportion to illumination intensity. Acute silencing of orexin neurons in vivo during the day (the inactive period) induced synchronization of the electroencephalogram and a reduction in amplitude of the electromyogram that is characteristic of slow-wave sleep (SWS). In contrast, orexin neuron photoinhibition was ineffective during the night (active period). Acute photoinhibition of orexin neurons during the day in orexin/Halo mice also reduced discharge of neurons in an orexin terminal field, the dorsal raphe (DR) nucleus. However, serotonergic DR neurons exhibited normal discharge rates in mice lacking orexin neurons. Thus, although usually highly dependent on orexin neuronal activity, serotonergic DR neuronal activity can be regulated appropriately in the chronic absence of orexin input. Together, these results demonstrate that acute inhibition of orexin neurons results in time-of-day-dependent induction of SWS and in reduced firing rate of neurons in an efferent projection site thought to be involved in arousal state regulation. The results presented here advance our understanding of the role of orexin neurons in the regulation of sleep/wakefulness and may be relevant to the mechanisms that underlie symptom progression in narcolepsy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
20
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10529-39
pubmed:meshHeading
pubmed-meshheading:21775598-Action Potentials, pubmed-meshheading:21775598-Analysis of Variance, pubmed-meshheading:21775598-Animals, pubmed-meshheading:21775598-Brain, pubmed-meshheading:21775598-Brain Waves, pubmed-meshheading:21775598-Color, pubmed-meshheading:21775598-Electroencephalography, pubmed-meshheading:21775598-Electromyography, pubmed-meshheading:21775598-Female, pubmed-meshheading:21775598-Green Fluorescent Proteins, pubmed-meshheading:21775598-Halorhodopsins, pubmed-meshheading:21775598-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:21775598-Male, pubmed-meshheading:21775598-Membrane Potentials, pubmed-meshheading:21775598-Mice, pubmed-meshheading:21775598-Mice, Transgenic, pubmed-meshheading:21775598-Neural Inhibition, pubmed-meshheading:21775598-Neurons, pubmed-meshheading:21775598-Neuropeptides, pubmed-meshheading:21775598-Sleep, pubmed-meshheading:21775598-Sodium Channel Blockers, pubmed-meshheading:21775598-Spectrum Analysis, pubmed-meshheading:21775598-Tetrodotoxin, pubmed-meshheading:21775598-Wakefulness
pubmed:year
2011
pubmed:articleTitle
Acute optogenetic silencing of orexin/hypocretin neurons induces slow-wave sleep in mice.
pubmed:affiliation
Division of Cell Signaling, National Institute for Physiological Sciences, Okazaki 444-8787, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural