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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0017428,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0162326,
umls-concept:C0185027,
umls-concept:C0185117,
umls-concept:C0205245,
umls-concept:C0441635,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C1979844,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
12
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pubmed:dateCreated |
1991-1-8
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pubmed:abstractText |
The entire 33-kb coding region of the mouse Na,K-ATPase alpha 1 subunit gene was cloned in two overlapping cosmids which contain inserts of 40 kb. To assess the functional expression of the mouse alpha 1 gene, the two cosmids were cotransfected into ouabain-sensitive CV-1 monkey cells yielding an average of 64 resistant colonies per 10(6) cells per microgram of DNA. Analysis of the DNA transferred to the ouabain-resistant transformants by the two cosmids suggests that the generation of a functional gene can occur by homologous recombination between the two introduced segments, as demonstrated by generation of a novel diagnostic restriction fragment. The ability to reconstruct the intact mouse alpha 1 gene in a heterologous host cell and to monitor its functional expression with a selection protocol permits direct identification and isolation of regulatory sequences for the gene.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-166393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-1970326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-2550800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-2694931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-2823111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-2853965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-2885848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3028470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3031668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3035563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3036582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3039660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3477547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3990687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-3994653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-4276295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-4434471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-454358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-6289898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-6294829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-6310370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2174112-6576360
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6619-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2174112-Animals,
pubmed-meshheading:2174112-Blotting, Southern,
pubmed-meshheading:2174112-Cell Line,
pubmed-meshheading:2174112-Cloning, Molecular,
pubmed-meshheading:2174112-Cosmids,
pubmed-meshheading:2174112-DNA,
pubmed-meshheading:2174112-Drug Resistance,
pubmed-meshheading:2174112-Gene Expression,
pubmed-meshheading:2174112-Genomic Library,
pubmed-meshheading:2174112-Mice,
pubmed-meshheading:2174112-Mice, Inbred Strains,
pubmed-meshheading:2174112-Nucleic Acid Hybridization,
pubmed-meshheading:2174112-Ouabain,
pubmed-meshheading:2174112-Restriction Mapping,
pubmed-meshheading:2174112-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:2174112-Transfection
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pubmed:year |
1990
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pubmed:articleTitle |
Functional expression of the genomic DNA sequences encoding mouse Na,K-ATPase alpha 1 gene by cotransfection of overlapping genomic DNA segments.
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pubmed:affiliation |
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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